Document Detail


Placental mRNA in maternal plasma as a predictor of ectopic pregnancy.
MedLine Citation:
PMID:  22342059     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To measure and compare placental mRNA expression in the maternal circulation among women with intrauterine and ectopic pregnancies.
METHODS: Plasma was collected from patients in early pregnancy at risk of ectopic pregnancy. Clinical information was prospectively collected and entered into a dedicated database. mRNA was isolated from maternal plasma and quantitative RT-PCR was performed to measure mRNA for human gonadotropin (hCG) and human placental lactogen (hPL). GAPDH mRNA expression was used as an internal control.
RESULTS: Twelve women with ectopic pregnancy and 13 women with intrauterine pregnancy were enrolled. Patients with ectopic pregnancy were 6 times more likely to have undetectable levels of hPL mRNA (relative risk [RR] 6.36; 95% confidence interval [CI], 1.70-23.20; P<0.01). They were also 8 times more likely to have undetectable levels of hCG mRNA (RR 8.64, 95% CI, 1.30-57.10; P<0.01). mRNA copy numbers for hPL and hCG (normalized by GAPDH) were significantly lower in the ectopic group than in the intrauterine group.
CONCLUSION: Placental mRNA is present in the maternal circulation in significantly lower copies in cases of ectopic pregnancy compared with cases of intrauterine pregnancy. Measurement of placental mRNA in the maternal circulation may help to distinguish between intrauterine and ectopic pregnancies.
Authors:
Peter Takacs; Sindy Jaramillo; Ram Datar; Anthony Williams; Joseph Olczyk; Kurt Barnhart
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-02-17
Journal Detail:
Title:  International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics     Volume:  117     ISSN:  1879-3479     ISO Abbreviation:  Int J Gynaecol Obstet     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-04-16     Completed Date:  2012-08-03     Revised Date:  2014-01-20    
Medline Journal Info:
Nlm Unique ID:  0210174     Medline TA:  Int J Gynaecol Obstet     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  131-3     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 International Federation of Gynecology and Obstetrics. Published by Elsevier Ireland Ltd. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Adult
Chorionic Gonadotropin / blood,  genetics
Female
Glyceraldehyde-3-Phosphate Dehydrogenases / blood,  genetics
Humans
Placenta / metabolism*
Placental Lactogen / blood,  genetics
Pregnancy
Pregnancy, Ectopic / blood,  diagnosis*
Prospective Studies
RNA, Messenger / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Young Adult
Grant Support
ID/Acronym/Agency:
K24 HD060687/HD/NICHD NIH HHS; R01 HD036455-10/HD/NICHD NIH HHS; R01-HD036455/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Chorionic Gonadotropin; 0/RNA, Messenger; 9035-54-5/Placental Lactogen; EC 1.2.1.-/Glyceraldehyde-3-Phosphate Dehydrogenases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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