Document Detail

Placental localization and expression of the cell death factors BNip3 and Nix in preeclampsia, intrauterine growth retardation and HELLP syndrome.
MedLine Citation:
PMID:  16219518     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: BNip3 and its homologue Nix are pro-apoptotic factors of the Bcl-2-family and are expressed in malignant tumors. In vitro, this expression was shown to be mediated by hypoxia. Recently, it has been shown that placental hypoxia as well as apoptosis are pathogenetic factors for pregnancy-induced hypertensive diseases and intrauterine growth retardation (IUGR). The aim of the study was to analyze placental expression of BNip3 and Nix in pregnancies complicated by preeclampsia, hemolysis, elevated liver enzymes and low platelets (HELLP) syndrome and IUGR. MATERIAL AND METHODS: Placental tissue was sampled from 10 pregnancies each with preeclampsia, HELLP syndrome, IUGR and gestational age-matched controls. The placental expression of BNip3/Nix has been investigated with immunohistochemistry by the use of specific human BNip3/Nix antibodies. RESULTS: In cytotrophoblastic cells, the BNip3 expression was strong in the control placentas, but only mediate in the placentas from pregnancies with preeclampsia, IUGR or HELLP syndrome. The intensity of the Nix staining showed a similar pattern. In the syncytiotrophoblast, there was a weak BNip3 staining observable in the control as well as IUGR samples, whereas BNip3 was undetectable in preeclamptic placentas or those with HELLP syndrome. For Nix, only in the preeclampsia a weak staining was detectable, whereas all other probes were negative. CONCLUSIONS: Our study shows for the first time that the pro-apoptotic proteins BNip3 and Nix are expressed in the human placenta. Pregnancies with placental dysfunction and hypertensive pregnancy disorders with different clinical manifestations are characterized by a significantly decreased expression of BNip3 and Nix. These results suggest that the hypothesis of generally increased placental apoptosis in pregnancy-induced hypertensive disorders caused by disturbed trophoblast invasion has to be partly reconsidered.
H Stepan; C Leo; S Purz; M Höckel; L-C Horn
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of obstetrics, gynecology, and reproductive biology     Volume:  122     ISSN:  0301-2115     ISO Abbreviation:  Eur. J. Obstet. Gynecol. Reprod. Biol.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-10-12     Completed Date:  2006-01-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375672     Medline TA:  Eur J Obstet Gynecol Reprod Biol     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  172-6     Citation Subset:  IM    
Department of Obstetrics and Gynecology, University of Leipzig, Philipp-Rosenthalstr. 55, 04103 Leipzig, Germany.
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MeSH Terms
Fetal Growth Retardation / metabolism,  pathology
HELLP Syndrome / metabolism,  pathology
Membrane Proteins / metabolism*
Placenta / metabolism*,  pathology
Pre-Eclampsia / metabolism,  pathology
Pregnancy Complications / metabolism*,  pathology*
Proto-Oncogene Proteins / metabolism*
Tumor Suppressor Proteins / metabolism*
Reg. No./Substance:
0/BNIP3 protein, human; 0/BNIP3L protein, human; 0/Membrane Proteins; 0/Proto-Oncogene Proteins; 0/Tumor Suppressor Proteins

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