Document Detail


Placental development during early pregnancy in sheep: effects of embryo origin on fetal and placental growth and global methylation.
MedLine Citation:
PMID:  23117132     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The origin of embryos including those created through assisted reproductive technologies might have profound effects on placental and fetal development, possibly leading to compromised pregnancies associated with poor placental development. To determine the effects of embryo origin on fetal size, and maternal and fetal placental cellular proliferation and global methylation, pregnancies were achieved through natural mating (NAT), or transfer of embryos generated through in vivo (NAT-ET), IVF, or in vitro activation (IVA). On Day 22 of pregnancy, fetuses were measured and placental tissues were collected to immunologically detect Ki67 (a marker of proliferating cells) and 5-methyl cytosine followed by image analysis, and determine mRNA expression for three DNA methyltransferases. Fetal length and labeling index (proportion of proliferating cells) in maternal caruncles (maternal placenta) and fetal membranes (fetal placenta) were less (P < 0.001) in NAT-ET, IVF, and IVA than in NAT. In fetal membranes, expression of 5-methyl cytosine was greater (P < 0.02) in IVF and IVA than in NAT. In maternal caruncles, mRNA expression for DNMT1 was greater (P < 0.01) in IVA compared with the other groups, but DNMT3A expression was less (P < 0.04) in NAT-ET and IVA than in NAT. In fetal membranes, expression of mRNA for DNMT3A was greater (P < 0.01) in IVA compared with the other groups, and was similar in NAT, NAT-ET, and IVF groups. Thus, embryo origin might have specific effects on growth and function of ovine uteroplacental and fetal tissues through regulation of tissue growth, DNA methylation, and likely other mechanisms. These data provide a foundation for determining expression of specific factors regulating placental and fetal tissue growth and function in normal and compromised pregnancies, including those achieved with assisted reproductive technologies.
Authors:
Anna T Grazul-Bilska; Mary Lynn Johnson; Pawel P Borowicz; Loren Baranko; Dale A Redmer; Lawrence P Reynolds
Publication Detail:
Type:  Journal Article; Randomized Controlled Trial; Research Support, American Recovery and Reinvestment Act; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-10-30
Journal Detail:
Title:  Theriogenology     Volume:  79     ISSN:  1879-3231     ISO Abbreviation:  Theriogenology     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2012-12-11     Completed Date:  2013-05-28     Revised Date:  2014-01-09    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  94-102     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Animals
DNA Methylation / physiology*
Embryo Transfer / veterinary
Embryo, Mammalian / cytology*,  metabolism,  physiology
Estrus Synchronization / genetics,  metabolism
Female
Fertilization in Vitro / veterinary
Fetal Development / genetics,  physiology*
Gestational Age
Male
Placenta / growth & development,  metabolism*
Placentation / genetics,  physiology*
Pregnancy
Pregnancy, Animal* / genetics,  metabolism
Sheep / embryology,  genetics,  metabolism,  physiology*
Grant Support
ID/Acronym/Agency:
HL64141/HL/NHLBI NIH HHS; R01 HL064141/HL/NHLBI NIH HHS
Comments/Corrections

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