| Placental development during early pregnancy in sheep: Effects of embryo origin on fetal and placental growth and global methylation. | |
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MedLine Citation:
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PMID: 23117132 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The origin of embryos including those created through assisted reproductive technologies might have profound effects on placental and fetal development, possibly leading to compromised pregnancies associated with poor placental development. To determine the effects of embryo origin on fetal size, and maternal and fetal placental cellular proliferation and global methylation, pregnancies were achieved through natural mating (NAT), or transfer of embryos generated through in vivo (NAT-ET), IVF, or in vitro activation (IVA). On Day 22 of pregnancy, fetuses were measured and placental tissues were collected to immunologically detect Ki67 (a marker of proliferating cells) and 5-methyl cytosine followed by image analysis, and determine mRNA expression for three DNA methyltransferases. Fetal length and labeling index (proportion of proliferating cells) in maternal caruncles (maternal placenta) and fetal membranes (fetal placenta) were less (P < 0.001) in NAT-ET, IVF, and IVA than in NAT. In fetal membranes, expression of 5-methyl cytosine was greater (P < 0.02) in IVF and IVA than in NAT. In maternal caruncles, mRNA expression for DNMT1 was greater (P < 0.01) in IVA compared with the other groups, but DNMT3A expression was less (P < 0.04) in NAT-ET and IVA than in NAT. In fetal membranes, expression of mRNA for DNMT3A was greater (P < 0.01) in IVA compared with the other groups, and was similar in NAT, NAT-ET, and IVF groups. Thus, embryo origin might have specific effects on growth and function of ovine uteroplacental and fetal tissues through regulation of tissue growth, DNA methylation, and likely other mechanisms. These data provide a foundation for determining expression of specific factors regulating placental and fetal tissue growth and function in normal and compromised pregnancies, including those achieved with assisted reproductive technologies. |
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Authors:
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Anna T Grazul-Bilska; Mary Lynn Johnson; Pawel P Borowicz; Loren Baranko; Dale A Redmer; Lawrence P Reynolds |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-10-29 |
Journal Detail:
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Title: Theriogenology Volume: - ISSN: 1879-3231 ISO Abbreviation: Theriogenology Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-11-2 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0421510 Medline TA: Theriogenology Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Animal Sciences, Center for Nutrition and Pregnancy, North Dakota State University, Fargo, North Dakota, USA. Electronic address: Anna.Grazul-Bilska@ndsu.edu. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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