| Placental blood leukocytes are functional and phenotypically different than peripheral leukocytes during human labor. | |
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MedLine Citation:
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PMID: 19748682 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rupture of the fetal membranes during human labor is associated with an inflammatory process localized to the maternal-fetal interface. There is evidence that specific leukocytes subsets are attracted to the choriodecidua, and that after homing they condition a local inflammatory microenvironment, possibly being directly involved in rupture of the membranes. In this study our aim was to compare the phenotypes and function of leukocytes located in the placental intervillous blood with peripheral leukocytes obtained before or after labor, including expression of modulators of inflammation in these cells. Flow cytometry revealed that the proportion of CD14(+) cells is increased in intervillous blood, suggesting the participation of monocytes/macrophages during labor. Real time qRT-PCR showed that at term gestation and particularly during labor, placental blood leukocytes adopt a different expression pattern of pro-inflammatory cytokines than leukocytes in peripheral blood, including IL-1beta and IL-1RA. During labor, both placental and peripheral leukocytes increase their secretion of matrix metalloproteinase-9. Moreover, we showed that placental leukocytes respond differently than peripheral leukocytes to bacterial lipopolysaccharide, secreting differential amounts of TNF-alpha, IL-1beta and IL-6. Finally, a preliminary proteomic characterization of placental leukocytes revealed a significantly higher number of individual proteins than in peripheral leukocytes. Our results support the existence of selective subsets of leukocytes recruited to the maternal-fetal interface that may participate in the triggering of parturition. |
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Authors:
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Rodrigo Vega-Sanchez; Nardhy Gomez-Lopez; Arturo Flores-Pliego; Susana Clemente-Galvan; Guadalupe Estrada-Gutierrez; Alejandro Zentella-Dehesa; Rolando Maida-Claros; Jorge Beltran-Montoya; Felipe Vadillo-Ortega |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-09-12 |
Journal Detail:
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Title: Journal of reproductive immunology Volume: 84 ISSN: 1872-7603 ISO Abbreviation: J. Reprod. Immunol. Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2010-02-01 Completed Date: 2010-04-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8001906 Medline TA: J Reprod Immunol Country: Ireland |
Other Details:
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Languages: eng Pagination: 100-10 Citation Subset: IM |
Copyright Information:
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2009 Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
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Research Direction, Instituto Nacional de Perinatologia Isidro Espinosa de los Reyes, Mexico City, Mexico. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD14
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immunology Extraembryonic Membranes / immunology Female Humans Inflammation / immunology Interleukin 1 Receptor Antagonist Protein / immunology Interleukin-1beta / immunology Interleukin-6 / immunology Labor, Obstetric / immunology* Leukocytes / immunology* Macrophages / immunology Matrix Metalloproteinase 9 / immunology Monocytes / immunology Placenta / immunology* Polysaccharides, Bacterial / immunology Pregnancy Tumor Necrosis Factor-alpha / immunology |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD14; 0/Interleukin 1 Receptor Antagonist Protein; 0/Interleukin-1beta; 0/Interleukin-6; 0/Polysaccharides, Bacterial; 0/Tumor Necrosis Factor-alpha; EC 3.4.24.35/Matrix Metalloproteinase 9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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