Document Detail


Placental growth factor: a promising diagnostic biomarker for tubal ectopic pregnancy.
MedLine Citation:
PMID:  21047920     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
CONTEXT: Tubal ectopic pregnancy is common, but accurate diagnosis is difficult and costly. There is currently no serum test to differentiate tubal from intrauterine implantation, and an effective biomarker of ectopic pregnancy would be a major clinical advance.
OBJECTIVE: A key feature of successful intrauterine implantation is the establishment of a supportive vascular network, and this has been associated with the activity of placental growth factor (PIGF). We hypothesized that the local decidual environment facilitates PIGF-dependent angiogenesis and that this pathway is not active in tubal implantation. We aimed to determine whether tubal implantation is manifest by an attenuation of the normal trophoblast PIGF response and whether serum PIGF levels are different in ectopic compared with intrauterine pregnancy.
DESIGN AND SETTING: Tissue and serum analysis was done at a large United Kingdom teaching hospital.
PATIENTS: Tissue and sera were collected from gestation-matched pregnant women undergoing surgical termination of pregnancy (viable intrauterine) (n = 15), evacuation of uterus for embryonic missed miscarriage (nonviable intrauterine) (n = 10) and surgery for tubal ectopic pregnancy (n = 15).
INTERVENTIONS: Trophoblast was examined by immunohistochemistry and quantitative RT-PCR, and serum was analyzed by ELISA.
RESULTS: PIGF was localized to the cytotrophoblast cells. Expression of PIGF mRNA was reduced in trophoblast isolated from women with ectopic compared with intrauterine pregnancies (P < 0.05). Serum PIGF was undetectable in women with tubal ectopic pregnancies and reduced, or undetectable, in miscarriage compared with viable intrauterine pregnancies (P < 0.01).
CONCLUSIONS: Serum PIGF is a promising novel diagnostic biomarker for early pregnancy location and outcome, and large-scale studies are now required to determine its clinical utility.
Authors:
Andrew W Horne; Julie L V Shaw; Amanda Murdoch; Sarah E McDonald; Alistair R Williams; Henry N Jabbour; W Colin Duncan; Hilary O D Critchley
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-11-03
Journal Detail:
Title:  The Journal of clinical endocrinology and metabolism     Volume:  96     ISSN:  1945-7197     ISO Abbreviation:  J. Clin. Endocrinol. Metab.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-01-06     Completed Date:  2011-02-04     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  0375362     Medline TA:  J Clin Endocrinol Metab     Country:  United States    
Other Details:
Languages:  eng     Pagination:  E104-8     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Biological Markers / metabolism
Enzyme-Linked Immunosorbent Assay
Female
Humans
Immunohistochemistry
Placenta / metabolism*
Pregnancy
Pregnancy Proteins / genetics,  metabolism*
Pregnancy, Tubal / diagnosis*,  metabolism
RNA, Messenger / genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Trophoblasts / metabolism*
Grant Support
ID/Acronym/Agency:
G0600048//Medical Research Council; G0600048//Medical Research Council; G0600048(80179)//Medical Research Council; G0802808//Medical Research Council; G0802808(90914)//Medical Research Council; G1002033//Medical Research Council; SCD/02//Chief Scientist Office; //Chief Scientist Office
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Pregnancy Proteins; 0/RNA, Messenger; 144589-93-5/placenta growth factor
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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