Document Detail

Placental-derived mesenchyme influences chorionic gonadotropin and progesterone secretion of human embryonic stem cell-derived trophoblasts.
MedLine Citation:
PMID:  20601539     Owner:  NLM     Status:  MEDLINE    
Studies of early placental development in humans are difficult because of limitations on experimental material availability from the perimplantation period. We used a coculture system to determine the effects of various effector cell types on trophoblast differentiation. Enhanced green fluorescent protein (EGFP)-expressing H1 human embryonic stem cells were used in co-suspension with human term placental fibroblasts (TPFs) and dermal fibroblasts (CI2F) to form combination embryoid bodies (EBs), with the goal of recapitulating placental morphogenesis through incorporation of placental mesenchymal cells. Overall, the results demonstrated that when using mesenchymal cells for EB preparation from term placentas (TPF), combination EB-derived trophoblasts secrete higher levels of human chorionic gonadotropin (hCG) and progesterone compared to EBs made without effector cells, whereas there was no effect of dermal fibroblasts. This is due to the secretory activity of the EB-derived trophoblasts and not due to the number of differentiated trophoblasts per EB, demonstrating that nontrophoblast cells of the placenta can influence trophoblast endocrine activity.
Maria Giakoumopoulos; Leah M Siegfried; Svetlana V Dambaeva; Mark A Garthwaite; M Clay Glennon; Thaddeus G Golos
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-07-02
Journal Detail:
Title:  Reproductive sciences (Thousand Oaks, Calif.)     Volume:  17     ISSN:  1933-7205     ISO Abbreviation:  Reprod Sci     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-11     Completed Date:  2010-11-22     Revised Date:  2014-09-24    
Medline Journal Info:
Nlm Unique ID:  101291249     Medline TA:  Reprod Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  798-808     Citation Subset:  IM    
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MeSH Terms
Cell Communication*
Cell Differentiation*
Cells, Cultured
Chorionic Gonadotropin / secretion*
Coculture Techniques
Embryonic Stem Cells / secretion*
Fibroblasts* / metabolism
Green Fluorescent Proteins / biosynthesis,  genetics
Mesoderm / cytology*,  metabolism
Placenta / cytology*,  metabolism
Progesterone / secretion*
Time Factors
Trophoblasts / secretion*
Grant Support
C06 RR015459-01/RR/NCRR NIH HHS; C06 RR020141-01/RR/NCRR NIH HHS; HD T32 041921/HD/NICHD NIH HHS; HD038843/HD/NICHD NIH HHS; HD046919/HD/NICHD NIH HHS; P01 HD038843/HD/NICHD NIH HHS; P01 HD038843-07/HD/NICHD NIH HHS; P01 HD038843-08/HD/NICHD NIH HHS; P01 HD038843-09/HD/NICHD NIH HHS; P01 HD038843-10/HD/NICHD NIH HHS; P51 RR000167/RR/NCRR NIH HHS; P51 RR000167-41/RR/NCRR NIH HHS; P51 RR000167-42/RR/NCRR NIH HHS; P51 RR000167-43/RR/NCRR NIH HHS; P51 RR000167-44/RR/NCRR NIH HHS; P51 RR000167-45/RR/NCRR NIH HHS; P51 RR000167-46/RR/NCRR NIH HHS; P51 RR000167-48/RR/NCRR NIH HHS; P51 RR000167-49/RR/NCRR NIH HHS; P51 RR000167-50/RR/NCRR NIH HHS; R21 HD046919/HD/NICHD NIH HHS; R21 HD046919-01A2/HD/NICHD NIH HHS; R21 HD046919-02/HD/NICHD NIH HHS; RR020141-01/RR/NCRR NIH HHS; RR15459-01/RR/NCRR NIH HHS; T32 HD041921/HD/NICHD NIH HHS; T32 HD041921-06/HD/NICHD NIH HHS; T32 HD041921-07/HD/NICHD NIH HHS; T32 HD041921-08/HD/NICHD NIH HHS; UL1 RR025011/RR/NCRR NIH HHS; UL1 RR025011/RR/NCRR NIH HHS; UL1 RR025011-01/RR/NCRR NIH HHS; UL1 RR025011-02/RR/NCRR NIH HHS; UL1 RR025011-03/RR/NCRR NIH HHS; UL1 RR025011-04/RR/NCRR NIH HHS; UL1 RR025011-05/RR/NCRR NIH HHS
Reg. No./Substance:
0/Chorionic Gonadotropin; 0/enhanced green fluorescent protein; 147336-22-9/Green Fluorescent Proteins; 4G7DS2Q64Y/Progesterone

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