Document Detail


Piwi-interacting RNAs protect DNA against loss during Oxytricha genome rearrangement.
MedLine Citation:
PMID:  23217708     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Genome duality in ciliated protozoa offers a unique system to showcase their epigenome as a model of inheritance. In Oxytricha, the somatic genome is responsible for vegetative growth, whereas the germline contributes DNA to the next sexual generation. Somatic nuclear development removes all transposons and other so-called "junk" DNA, which comprise ~95% of the germline. We demonstrate that Piwi-interacting small RNAs (piRNAs) from the maternal nucleus can specify genomic regions for retention in this process. Oxytricha piRNAs map primarily to the somatic genome, representing the ~5% of the germline that is retained. Furthermore, injection of synthetic piRNAs corresponding to normally deleted regions leads to their retention in later generations. Our findings highlight small RNAs as powerful transgenerational carriers of epigenetic information for genome programming.
Authors:
Wenwen Fang; Xing Wang; John R Bracht; Mariusz Nowacki; Laura F Landweber
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Cell     Volume:  151     ISSN:  1097-4172     ISO Abbreviation:  Cell     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-10     Completed Date:  2013-02-06     Revised Date:  2013-12-12    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1243-55     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/JN604928;  JN604929;  JN604930;  JN604931;  JN604932;  JN604933;  JN604934;  JN604935;  JN604936;  JN604937;  JN604938;  JN604939;  JN604940; GEO/GSE35018;  GSE40081
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Base Sequence
Conjugation, Genetic*
Gene Rearrangement
Genome, Protozoan*
Macronucleus / genetics
Molecular Sequence Data
Oxytricha / cytology*,  genetics*
Phylogeny
RNA, Protozoan / genetics*
RNA, Small Interfering / genetics*
Sequence Alignment
Grant Support
ID/Acronym/Agency:
260358//European Research Council; F32 GM099462/GM/NIGMS NIH HHS; GM59708/GM/NIGMS NIH HHS; R01 GM059708/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Protozoan; 0/RNA, Small Interfering
Comments/Corrections
Comment In:
Cell. 2012 Dec 7;151(6):1157-8   [PMID:  23217700 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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