| Piwi-Interacting RNAs Protect DNA against Loss during Oxytricha Genome Rearrangement. | |
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MedLine Citation:
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PMID: 23217708 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Genome duality in ciliated protozoa offers a unique system to showcase their epigenome as a model of inheritance. In Oxytricha, the somatic genome is responsible for vegetative growth, whereas the germline contributes DNA to the next sexual generation. Somatic nuclear development removes all transposons and other so-called "junk" DNA, which comprise ∼95% of the germline. We demonstrate that Piwi-interacting small RNAs (piRNAs) from the maternal nucleus can specify genomic regions for retention in this process. Oxytricha piRNAs map primarily to the somatic genome, representing the ∼5% of the germline that is retained. Furthermore, injection of synthetic piRNAs corresponding to normally deleted regions leads to their retention in later generations. Our findings highlight small RNAs as powerful transgenerational carriers of epigenetic information for genome programming. |
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Authors:
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Wenwen Fang; Xing Wang; John R Bracht; Mariusz Nowacki; Laura F Landweber |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cell Volume: 151 ISSN: 1097-4172 ISO Abbreviation: Cell Publication Date: 2012 Dec |
Date Detail:
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Created Date: 2012-12-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0413066 Medline TA: Cell Country: United States |
Other Details:
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Languages: eng Pagination: 1243-55 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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