| Pivotal karyometric measurements in different types of cardiomyopathic morphology: study of hearts explanted from transplant recipients. | |
| | |
MedLine Citation:
|
PMID: 19857705 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
BACKGROUND: Morphometric studies based on the measurement of cardiocyte nuclei have focused on progressive hypertrophy rather than shape, which is a deciding factor for the diagnosis of hypertrophy in myocardial diseases. The aim of this research was to demonstrate how the digital morphology of cardiocyte nuclei change correlated with the type of myocardial pathology. MATERIALS AND METHODS: The study groups encompassed 7 hearts with dilated cardiomyopathy (DCM) and 8 hearts with ischemic heart disease (IHD) which were explanted. A comparative group consisting of myocardial hypertrophy was contrasted with a control group of donor heart fragments. Cardiocyte nuclei were evaluated morphometrically on histologic slides. We calculated the nuclear area, length, breadth, perimeter, roundness, elongation, fullness factors, and nuclear chromatin mean gray level. The results were subjected to discriminant analysis. RESULTS: All karyometric measurements analyzed by backward discriminant analysis showed only 2 powerful factors: nuclear breadth and chromatin mean gray level. The Mahalanobis distance showed the proximity of control and hypertrophy groups, whereas differences between IHD and DCM were nonsignificant. CONCLUSION: The lack of karyometric differences between IHD and DCM suggested a common morphologic response for long-lasting progressive injury. The main morphologic differences were dependent on nuclear chromatin activity/stainability and nuclear breadth, suggesting darker and thinned nuclei in normal and adaptative stages and irregular brighter nuclei in cardiomyopathies. |
| | |
Authors:
|
J Nozyński; M Zakliczyński; D Konecka-Mrówka; S Zegleń; R Przybylski; M Zembala; D Lange; E Zembala-Nozyńska; T Mecik-Kronenberg; K Dabrówka |
Related Documents
:
|
16165015 - Physiologic growth and pathologic genes in cardiac development and cardiomyopathy. 19190955 - Ischemic injury activates pthrp and pth1r expression in human ventricular cardiomyocytes. 18534575 - Comparison of pharmacodynamics between carvedilol and metoprolol in rats with isoproter... 10830785 - Metallothionein inhibits myocardial apoptosis in copper-deficient mice: role of atrial ... 20228415 - Pharmacological inhibition of leukotriene biosynthesis: effects on the heart conductance. 9572115 - Left-right development in xenopus and zebrafish. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Transplantation proceedings Volume: 41 ISSN: 1873-2623 ISO Abbreviation: Transplant. Proc. Publication Date: 2009 Oct |
Date Detail:
|
Created Date: 2009-10-27 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0243532 Medline TA: Transplant Proc Country: United States |
Other Details:
|
Languages: eng Pagination: 3179-84 Citation Subset: IM |
Affiliation:
|
Silesian Centre for Heart Diseases, 41-800 Zabrze, Poland. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Differences in antiapoptotic, proliferative activities and morphometry in dilated and ischemic cardi...
Next Document: Different role of advanced glycation end products in pathology of transplanted heart in patients wit...