Document Detail


Pituitary tumor transforming gene (PTTG) regulates placental JEG-3 cell division and survival: evidence from live cell imaging.
MedLine Citation:
PMID:  10935539     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The pituitary transforming gene, PTTG, is abundantly expressed in endocrine neoplasms. PTTG has recently been recognized as a mammalian securin based on its biochemical homology to Pds1p. PTTG expression and intracellular localization were therefore studied during the cell cycle in human placental JEG-3 cells. PTTG mRNA and protein expressions were low at the G1/S border, gradually increased during S phase, and peaked at G2/M, but PTTG levels were attenuated as cells entered G1. In interphase cells, wild-type PTTG, an epitope-tagged PTTG, and a PTTG-EGFP conjugate all localized to both the nucleus and cytoplasm, but in mitotic cells, PTTG was not observed in the chromosome region. PTTG-EGFP colocalized with mitotic spindles in early mitosis and was degraded in anaphase. Intracellular fates of PTTG-EGFP and a conjugate of EGFP and a mutant inactivated PTTG devoid of an SH3-binding domain were observed by real-time visualization of the EGFP conjugates in live cells. The same cells were continuously observed as they progressed from G1/S border to S, G2/M, and G1. Most cells (67%) expressing PTTG-EGFP died by apoptosis, and few cells (4%) expressing PTTG-EGFP divided, whereas those expressing mutant PTTG-EGFP divided. PTTG-EGFP, as well as the mutant PTTG-EGFP, disappeared after cells divided. The results show that PTTG expression and localization are cell cycle-dependent and demonstrate that PTTG regulates endocrine tumor cell division and survival.
Authors:
R Yu; S G Ren; G A Horwitz; Z Wang; S Melmed
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular endocrinology (Baltimore, Md.)     Volume:  14     ISSN:  0888-8809     ISO Abbreviation:  Mol. Endocrinol.     Publication Date:  2000 Aug 
Date Detail:
Created Date:  2001-01-04     Completed Date:  2001-01-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8801431     Medline TA:  Mol Endocrinol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1137-46     Citation Subset:  IM    
Affiliation:
Division of Endocrinology, Cedars-Sinai Research Institute-UCLA School of Medicine, Los Angeles, California 90048, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Cell Cycle / physiology*
Cell Division / physiology
Cell Survival / physiology
Green Fluorescent Proteins
Humans
Luminescent Proteins / analysis,  genetics,  metabolism
Molecular Sequence Data
Neoplasm Proteins / genetics,  metabolism*
Placenta / cytology*
Recombinant Proteins / genetics,  metabolism
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
CA-75979/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Luminescent Proteins; 0/Neoplasm Proteins; 0/Recombinant Proteins; 0/pituitary tumor-transforming proteins; 147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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