| Pituitary hollow fiber units in vivo and in vitro. | |
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MedLine Citation:
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PMID: 7242858 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A new technique is described for the study of mammalian pituitary cells in vivo and in vitro. The method involves encapsulation of freshly trypsinized rat, sheep or human pituitary cells in XM-50 Amicon hollow fibers followed by their intracranial implantation into hypophysectomized rats. These pituitary fiber units promoted recipient growth for 3 weeks before weight gains plateaued. Body composition analyses showed that significant quantities of protein, fat and ash accounted for the weight gain. Morphological study of the capsule contents 7-39 days postimplantation revealed the presence of intact somatotrophs and corticotrophs. The hollow fibers may provide an immunologically privileged site by virtue of the fact that the 50,000 dalton pores making up the lumen surface permit pituitary hormones to diffuse from the capsule, but theoretically do not permit immunoglobulin molecules to penetrate the capsule. Growth of hypox rats receiving capsules containing allogeneic rat pituitary cells, sheep cells or pieces of human postmortem pituitary support this concept. Furthermore, rats implanted with human PRL adenoma cells had detectable quantities of circulating hPRL 100 days postimplantation. It is suggested that the pituitary hollow fiber units function when they come in contact with a ventricular surface of a hypox animal. With these units, it will be possible to study function of the same group of pituitary cells in vitro and in vivo. |
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Authors:
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W C Hymer; D L Wilbur; R Page; E Hibbard; R C Kelsey; J M Hatfield |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Neuroendocrinology Volume: 32 ISSN: 0028-3835 ISO Abbreviation: Neuroendocrinology Publication Date: 1981 Jun |
Date Detail:
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Created Date: 1981-08-27 Completed Date: 1981-08-27 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0035665 Medline TA: Neuroendocrinology Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 339-49 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acrylic Resins* Adult Aged Animals Body Composition Female Growth Hormone / metabolism Humans Hypophysectomy Male Microscopy, Electron, Scanning Organ Size Pituitary Gland / transplantation* Polyvinyl Chloride* Polyvinyls* Prolactin / metabolism Rats / growth & development* Sheep Transplantation, Heterologous Transplantation, Homologous |
| Grant Support | |
ID/Acronym/Agency:
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CA 23248/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Acrylic Resins; 0/Polyvinyls; 55963-89-8/XM-50 Amicon; 9002-62-4/Prolactin; 9002-72-6/Growth Hormone; 9002-86-2/Polyvinyl Chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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