| Pituitary folliculo-stellate-like cell line (TtT/GF) responds to novel hypophysiotropic peptide (pituitary adenylate cyclase-activating peptide), showing increased adenosine 3',5'-monophosphate and interleukin-6 secretion and cell proliferation. | |
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MedLine Citation:
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PMID: 8404665 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Several studies have shown that folliculo-stellate cells (FS cells) in the anterior pituitary gland exhibit paracrine functions. Recently, we established a pituitary FS-like cell line, TtT/GF, which was derived from an isologously transplantable pituitary thyrotropic tumor line induced by radiothyroidectomy. In studies to examine the function of FS cells, we found that two forms of a novel hypophysiotropic peptide, pituitary adenylate cyclase-activating polypeptide (PACAP), were potent activators of TtT/GF cells. Both the 27- and 38-amino acid forms of PACAP (PACAP-27 and PACAP-38) and vasoactive intestinal peptide (VIP) increased the levels of cAMP in TtT/GF cells in a similar dose-dependent manner. PACAP-27 and PACAP-38 specifically stimulated the proliferation of TtT/GF cells dose dependently, whereas VIP was ineffective. The minimal effective concentration of the PACAPs inducing cell proliferation was between 10(-8)-10(-7) M. However, PACAP-27 was much less potent than PACAP-38 in stimulating cell proliferation and DNA synthesis. PACAP-38, PACAP-27, and VIP all stimulated the release of interleukin-6 (IL-6) from TtT/GF cells. PACAP 38 (10(-8) M) stimulated IL-6 production effectively within 1 h of incubation, and the level attained at 8 h of cultivation (620 pg/ml) was nearly 10-fold that in the absence of PACAP-38 (60 pg/ml). PACAP-38 and VIP stimulated IL-6 secretion significantly at 10(-10)-10(-9) M in a bell-shaped manner; the maximum values were 10(-7) and 10(-8) M, respectively. On the other hand, IL-6 secretion stimulated by PACAP-27 became saturated at 10(-8) M, and the maximum value (320 pg/ml) was about 25% of that stimulated by PACAP-38 (1280 pg/ml). These findings obtained using TtT/GF cells as a model of FS cells suggest that PACAP acts as a hypophysiotropic factor, which targets FS cells and stimulates their proliferation, adenylate cyclase activation, and IL-6 secretion. |
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Authors:
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H Matsumoto; C Koyama; T Sawada; K Koike; K Hirota; A Miyake; A Arimura; K Inoue |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Endocrinology Volume: 133 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 1993 Nov |
Date Detail:
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Created Date: 1993-11-19 Completed Date: 1993-11-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2150-5 Citation Subset: AIM; IM |
Affiliation:
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Discovery Research Laboratories I, Takeda Chemical Industries, Ltd., Ibaraki, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Division / drug effects* Cyclic AMP / metabolism* DNA / biosynthesis Interleukin-6 / secretion* Mice Neuropeptides / pharmacology* Pituitary Adenylate Cyclase-Activating Polypeptide Pituitary Gland, Anterior / cytology, drug effects, metabolism* Pituitary Neoplasms Tumor Cells, Cultured Vasoactive Intestinal Peptide / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Adcyap1 protein, mouse; 0/Interleukin-6; 0/Neuropeptides; 0/Pituitary Adenylate Cyclase-Activating Polypeptide; 37221-79-7/Vasoactive Intestinal Peptide; 60-92-4/Cyclic AMP; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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