Document Detail

Pituitary adenylate cyclase-activating polypeptide dilates cerebral arterioles of newborn pigs.
MedLine Citation:
PMID:  8390692     Owner:  NLM     Status:  MEDLINE    
The actions of the 38- and 27-amino acid forms of synthetic pituitary adenylate cyclase-activating polypeptide (PACAP-38 and PACAP-27) on cerebral arterioles were tested in anesthetized newborn pigs equipped with closed cranial windows. The diameter changes of pial arterioles to topical PACAP were measured and cortical periarachnoid cerebrospinal fluid samples were collected for measurement of cAMP. The 38- and 27-amino acid forms of PACAP produced similar dose-dependent vasodilations. The increases of pial arteriolar diameter produced by PACAP-38 were 6 +/- 1%, 15 +/- 2%, 23 +/- 3%, and 38 +/- 3%, and those produced by PACAP-27 were 6 +/- 1%, 15 +/- 2%, 27 +/- 5%, and 38 +/- 8% at 10((-9), 10(-8), 10(-7), and 10(-6) M, respectively. Arteriolar diameter began to increase 1-2 min and reached a maximum 6-8 min after topical application of PACAP. Vasoactive concentrations of PACAP-38 and PACAP-27 increased cerebrospinal fluid cAMP levels dose dependently. Thus, PACAP-38 and PACAP-27 appear to stimulate cerebral adenylate cyclase and are potent dilators of newborn pigs pial arterioles.
S Tong; H Parfenova; M Shibata; S Zuckerman; W M Armstead; C W Leffler
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)     Volume:  203     ISSN:  0037-9727     ISO Abbreviation:  Proc. Soc. Exp. Biol. Med.     Publication Date:  1993 Jul 
Date Detail:
Created Date:  1993-07-16     Completed Date:  1993-07-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7505892     Medline TA:  Proc Soc Exp Biol Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  343-7     Citation Subset:  IM    
Department of Physiology and Biophysics, University of Tennessee, Memphis 38163.
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MeSH Terms
Animals, Newborn
Arterioles / drug effects,  physiology
Brain / blood supply*
Cyclic AMP / cerebrospinal fluid
Neuropeptides / administration & dosage,  pharmacology*
Pia Mater / blood supply
Pituitary Adenylate Cyclase-Activating Polypeptide
Vasodilation / drug effects*
Grant Support
Reg. No./Substance:
0/Neuropeptides; 0/Pituitary Adenylate Cyclase-Activating Polypeptide; 60-92-4/Cyclic AMP

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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