| Pituitary adenylate cyclase-activating peptide (PACAP) stimulates production of interleukin-6 in rat Müller cells. | |
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MedLine Citation:
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PMID: 16427158 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Pituitary adenylate cyclase-activating peptide (PACAP) is known to regulate not only neurons but also astrocytes. Here, we investigated, both in vitro and in vivo, the effects of PACAP38 on rat Müller cells, which are the predominant glial element in the retina. Müller cells isolated from juvenile Wistar rats were treated with PACAP38 or PACAP6-38, a PACAP selective antagonist. Cell proliferation was determined by measuring the incorporation of bromodeoxyuridine with ELISA. Interleukin-6 (IL-6) levels in the culture medium were determined by a bioassay using B9 cells, IL-6 dependent hybridoma. In adult Wistar rats, the expression of IL-6 in the retina after intravitreal injection of PACAP38 (10 pmol) was assessed by immunohistochemistry. PACAP38 stimulated IL-6 production in Müller cells at a concentration as low as 10(-12) M, which did not induce cell proliferation. This elevation of IL-6 production was inhibited by PACAP6-38. Radial IL-6 expression was observed throughout the retina at 2 and 3 days after PACAP38 injection. These data demonstrate that Müller cells are one of the target cells for PACAP. IL-6, which is released from Müller cells with stimulation by PACAP, may play a significant role in the retina. |
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Authors:
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Masayoshi Nakatani; Tamotsu Seki; Yuko Shinohara; Chisato Taki; Shigeru Nishimura; Atsushi Takaki; Seiji Shioda |
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Publication Detail:
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Type: Journal Article Date: 2006-01-19 |
Journal Detail:
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Title: Peptides Volume: 27 ISSN: 0196-9781 ISO Abbreviation: Peptides Publication Date: 2006 Jul |
Date Detail:
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Created Date: 2006-06-19 Completed Date: 2006-09-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8008690 Medline TA: Peptides Country: United States |
Other Details:
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Languages: eng Pagination: 1871-6 Citation Subset: IM |
Affiliation:
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Bioengineering Institute, Assessment Research Department, Nidek Co. Ltd., 6-209-1 Miyakitadori Gamagori-shi, Aichi 443-0022, Japan. masayoshi_nakatani@nidek.co.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Assay Cell Proliferation Cells, Cultured Dose-Response Relationship, Drug Enzyme-Linked Immunosorbent Assay Interleukin-6 / biosynthesis*, metabolism Male Neuroglia / cytology* Pituitary Adenylate Cyclase-Activating Polypeptide / metabolism, physiology* Rats Rats, Wistar Retina / cytology, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-6; 0/Pituitary Adenylate Cyclase-Activating Polypeptide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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