Document Detail

Pituitary adenylate cyclase-activating peptide (PACAP) stimulates production of interleukin-6 in rat Müller cells.
MedLine Citation:
PMID:  16427158     Owner:  NLM     Status:  MEDLINE    
Pituitary adenylate cyclase-activating peptide (PACAP) is known to regulate not only neurons but also astrocytes. Here, we investigated, both in vitro and in vivo, the effects of PACAP38 on rat Müller cells, which are the predominant glial element in the retina. Müller cells isolated from juvenile Wistar rats were treated with PACAP38 or PACAP6-38, a PACAP selective antagonist. Cell proliferation was determined by measuring the incorporation of bromodeoxyuridine with ELISA. Interleukin-6 (IL-6) levels in the culture medium were determined by a bioassay using B9 cells, IL-6 dependent hybridoma. In adult Wistar rats, the expression of IL-6 in the retina after intravitreal injection of PACAP38 (10 pmol) was assessed by immunohistochemistry. PACAP38 stimulated IL-6 production in Müller cells at a concentration as low as 10(-12) M, which did not induce cell proliferation. This elevation of IL-6 production was inhibited by PACAP6-38. Radial IL-6 expression was observed throughout the retina at 2 and 3 days after PACAP38 injection. These data demonstrate that Müller cells are one of the target cells for PACAP. IL-6, which is released from Müller cells with stimulation by PACAP, may play a significant role in the retina.
Masayoshi Nakatani; Tamotsu Seki; Yuko Shinohara; Chisato Taki; Shigeru Nishimura; Atsushi Takaki; Seiji Shioda
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Publication Detail:
Type:  Journal Article     Date:  2006-01-19
Journal Detail:
Title:  Peptides     Volume:  27     ISSN:  0196-9781     ISO Abbreviation:  Peptides     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-06-19     Completed Date:  2006-09-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008690     Medline TA:  Peptides     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1871-6     Citation Subset:  IM    
Bioengineering Institute, Assessment Research Department, Nidek Co. Ltd., 6-209-1 Miyakitadori Gamagori-shi, Aichi 443-0022, Japan.
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MeSH Terms
Biological Assay
Cell Proliferation
Cells, Cultured
Dose-Response Relationship, Drug
Enzyme-Linked Immunosorbent Assay
Interleukin-6 / biosynthesis*,  metabolism
Neuroglia / cytology*
Pituitary Adenylate Cyclase-Activating Polypeptide / metabolism,  physiology*
Rats, Wistar
Retina / cytology,  metabolism
Reg. No./Substance:
0/Interleukin-6; 0/Pituitary Adenylate Cyclase-Activating Polypeptide

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