Document Detail


Pitfalls with rapid HIV antibody testing in HIV-infected children in the Western Cape, South Africa.
MedLine Citation:
PMID:  16875874     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Rapid HIV antibody tests are commonly used for HIV diagnosis in the developing world. These tests are generally reported as sensitive, despite paucity of evaluations in paediatric populations. OBJECTIVES: We tested specimens of paediatric patients, known to be HIV-infected, to detect any false negative tests and determine associations with such an outcome. STUDY DESIGN: One hundred and fifty-three specimens, from 109 patients, recorded to be HIV-infected by standard testing, were tested on the Capillustrade mark HIV-1/HIV-2 test (Trinity Biotech, Ireland); 150 specimens also had sufficient volume to be tested on Abbott Determinetrade mark HIV1/2 assay (Abbott GmbH, Wiesbaden, Germany). Treatment information, CD4 counts and HIV-1 viral load measurements were obtained from patient files and laboratory databases. RESULTS: Twenty-one of 153 specimens tested negative on the Capillus (sensitivity 86.3%). False negative results by Capillus were associated with antiretroviral treatment (ART) (p=0.0018) and lower HIV-1 viral load (p=0.013). Serial dilutions of some of the specimens indicated that both rapid tests, and the Capillus in particular, became negative at lower dilutions than an HIV enzyme immunoassay (EIA). CONCLUSIONS: The Capillus test had an unexpectedly low sensitivity in a South African population of HIV-infected children that had access to antiretroviral treatment, posing a risk of false negative HIV testing.
Authors:
M Claassen; G U van Zyl; S N J Korsman; L Smit; M F Cotton; W Preiser
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Publication Detail:
Type:  Journal Article     Date:  2006-07-27
Journal Detail:
Title:  Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology     Volume:  37     ISSN:  1386-6532     ISO Abbreviation:  J. Clin. Virol.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-28     Completed Date:  2006-12-14     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9815671     Medline TA:  J Clin Virol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  68-71     Citation Subset:  IM    
Affiliation:
Discipline of Medical Virology, Department of Pathology, Faculty of Health Sciences, University of Stellenbosch and NHLS, Tygerberg 7505, South Africa.
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MeSH Terms
Descriptor/Qualifier:
AIDS Serodiagnosis*
Anti-HIV Agents / therapeutic use
CD4 Lymphocyte Count
False Negative Reactions
HIV Antibodies / blood*
HIV Infections / diagnosis*,  drug therapy
Humans
Infant
Infant, Newborn
Predictive Value of Tests
Sensitivity and Specificity
South Africa
Statistics as Topic
Viral Load
Chemical
Reg. No./Substance:
0/Anti-HIV Agents; 0/HIV Antibodies

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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