Document Detail


Pipette-friendly laminar flow patterning for cell-based assays.
MedLine Citation:
PMID:  21523269     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Laminar flow patterning (LFP) is a characteristic method of microfluidic systems that allows two (or more) different solutions to flow side-by-side in a channel without convective mixing. This fluid behavior can be used to pattern cell suspensions, particles, and treatments as well as to create chemical gradients. LFP is typically implemented using syringe pumps and, for this reason, is most effective in constant flow scenarios such as long-term gradient generation. However, the complexity of using syringe pumps for patterning cell suspensions typically makes it a less attractive option than other standard patterning methods. We present a passive microfluidic method that enables short-term LFP of multiple fluids using a single pipette and allows each sample to be loaded in any sequence, at any point in time relative to one another. The proposed method is well-suited for cell-based assays, reduces the complexity of LFP to be on a similar level as other cell patterning methods, can be scaled to include more than two streams of fluid, and enables arrays of individually addressable devices for LFP on a single chip.
Authors:
Erwin Berthier; Jay Warrick; Ben Casavant; David J Beebe
Related Documents :
3679529 - The safety of and the strain on the donor during thrombocytapheresis on the cell separa...
7409839 - Separation of viable from nonviable cells using a discontinuous density gradient.
2472759 - An automated analysis of chemotaxis in vitro using a computer-assisted scanning densito...
3876289 - Early rheumatoid-like synovial lesions in rabbits drinking cow's milk. i. joint pathology.
43269 - Isoelectric focusing of cells using zwitterionic buffers.
7258859 - Cybest-cdms; automated cell dispersion and monolayer smearing device for cybest.
17308979 - Liver-derived cell lines qsg-7701 and hepg2 support different hbv replication patterns.
17007929 - Methylmercury genotoxicity: a novel effect in human cell lines of the central nervous s...
8714689 - Effects of taxol on the polymerization and posttranslational modification of class iii ...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-04-26
Journal Detail:
Title:  Lab on a chip     Volume:  11     ISSN:  1473-0189     ISO Abbreviation:  Lab Chip     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-06-02     Completed Date:  2011-09-19     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  2060-5     Citation Subset:  IM    
Affiliation:
University of Wisconsin-Madison, Biomedical Engineering, 1111 Highland Av, Madison, WI 53705, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Biological Assay* / instrumentation
Cell Line, Tumor
Cell Movement
Humans
Microfluidics* / instrumentation,  methods
Models, Biological
Grant Support
ID/Acronym/Agency:
5T15LM007359/LM/NLM NIH HHS; R33 CA137673/CA/NCI NIH HHS; R33 CA137673/CA/NCI NIH HHS
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Effect of ionic strength and type of ions on the structure of water swollen polyelectrolyte multilay...
Next Document:  Microwave discharge electrodeless lamps (MDEL). Part VII. Photo-isomerization of trans-urocanic aci...