Document Detail

Pipette-friendly laminar flow patterning for cell-based assays.
MedLine Citation:
PMID:  21523269     Owner:  NLM     Status:  MEDLINE    
Laminar flow patterning (LFP) is a characteristic method of microfluidic systems that allows two (or more) different solutions to flow side-by-side in a channel without convective mixing. This fluid behavior can be used to pattern cell suspensions, particles, and treatments as well as to create chemical gradients. LFP is typically implemented using syringe pumps and, for this reason, is most effective in constant flow scenarios such as long-term gradient generation. However, the complexity of using syringe pumps for patterning cell suspensions typically makes it a less attractive option than other standard patterning methods. We present a passive microfluidic method that enables short-term LFP of multiple fluids using a single pipette and allows each sample to be loaded in any sequence, at any point in time relative to one another. The proposed method is well-suited for cell-based assays, reduces the complexity of LFP to be on a similar level as other cell patterning methods, can be scaled to include more than two streams of fluid, and enables arrays of individually addressable devices for LFP on a single chip.
Erwin Berthier; Jay Warrick; Ben Casavant; David J Beebe
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-04-26
Journal Detail:
Title:  Lab on a chip     Volume:  11     ISSN:  1473-0189     ISO Abbreviation:  Lab Chip     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-06-02     Completed Date:  2011-09-19     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  2060-5     Citation Subset:  IM    
University of Wisconsin-Madison, Biomedical Engineering, 1111 Highland Av, Madison, WI 53705, USA.
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MeSH Terms
Biological Assay* / instrumentation
Cell Line, Tumor
Cell Movement
Microfluidics* / instrumentation,  methods
Models, Biological
Grant Support
5T15LM007359/LM/NLM NIH HHS; R33 CA137673/CA/NCI NIH HHS; R33 CA137673/CA/NCI NIH HHS

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