| Pioglitazone attenuates endotoxin induced acute lung injury by reducing neutrophil recruitment. | |
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MedLine Citation:
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PMID: 22267758 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Treatment of acute lung injury (ALI) remains an unsolved problem in intensive care medicine. Activation and recruitment of neutrophils are regarded as key mechanisms in progression of ALI. As pioglitazone (pio) holds potent pleiotropic anti-inflammatory effects, we here explored its effects during ALI.C57Bl/6 mice were exposed to aerosolized LPS (500 μg·mL(-1)) and alveolar, interstitial, and intravascular neutrophils were assessed 4 h later. Lung permeability changes were evaluated by FITC-dextran clearance and protein content in the BAL fluid. In vitro, human isolated neutrophils were pre-treated with pio (1 or 3 hours, 10 μM) and then activated with fMLP. Neutrophil activation, adhesion, release and formation of reactive oxygen species and phagocytosis were measured thereafter.Pio treatment before or after induction of ALI significantly diminished alveolar (pio before LPS: reduction by 73 %; pio after LPS: reduction by 67 %) and interstitial neutrophil influx (pio before LPS: reduction by 55 %; pio after LPS: reduction by 63 %) and reduced lung permeability changes (pio before LPS: reduction by 64 %; pio after LPS: reduction by 58 %) indicating a protective role of pio in ALI. Moreover, pio significantly reduced degranulation and adhesion of neutrophils without affecting reactive oxygen formation and release or bacterial phagocytosis.Pio reduces recruitment and activation of neutrophils hereby protecting from LPS-induced ALI. Our results imply a potential role for pio in the management of ALI. |
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Authors:
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J Grommes; M Mörgelin; O Soehnlein |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-20 |
Journal Detail:
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Title: The European respiratory journal : official journal of the European Society for Clinical Respiratory Physiology Volume: - ISSN: 1399-3003 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-23 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8803460 Medline TA: Eur Respir J Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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* Institute for Molecular Cardiovascular Research (IMCAR). |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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