Document Detail


Pineal arylalkylamine N-acetyl-transferase (Aanat) gene expression as a target of inflammatory mediators in the chicken.
MedLine Citation:
PMID:  22935823     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Previously, we demonstrated that experimental peritonitis in chickens was attenuated by treatment with exogenous melatonin, while the developing inflammation decreased pineal AANAT activity. This suggested the existence of a bidirectional relationship between the activated immune system and pineal gland function. The aim of the present study was to identify the step(s) in the chicken pineal melatonin biosynthetic pathway that are affected by inflammation. Peritonitis was evoked by i.p. injection of thioglycollate solution, either 2h after the start, or 2h before the end of the light period, and the animals were sacrificed 4h later. The effect of inflammation on the expression of genes encoding enzymes participating in melatonin biosynthesis in the pineal gland, i.e. tryptophan hydroxylase 1 (Tph1), dopa decarboxylase (Ddc), arylalkylamine N-acetyl-transferase (Aanat) and acetylserotonin O-methyltransferase (Asmt), was evaluated by qPCR. The pineal and serum melatonin concentration as well as the content of its precursors in the pineal gland were measured, along with the activity of the relevant biosynthetic enzymes. Developing peritonitis caused an increase in the pineal levels of the Tph1 mRNA during the night and the Asmt mRNA during the day, while nocturnal Aanat transcription was reduced. Both the pineal and serum melatonin level and the pineal content of N-acetylserotonin (NAS) were decreased during the night in birds with peritonitis. The amount and activity of pineal AANAT were significantly reduced, while the activity of HIOMT was increased under these experimental conditions. These results indicate that the observed decrease in MEL biosynthesis in chickens with developing inflammation is a result of transcriptional downregulation of the Aanat gene, followed by reduced synthesis and activity of the encoded enzyme.
Authors:
Aneta Piesiewicz; Urszula Kedzierska; Iwona Adamska; Michal Usarek; Michal Zeman; Krystyna Skwarlo-Sonta; Pawel Marek Majewski
Related Documents :
8395003 - Synchronous expression of line-1 rna and protein in mouse embryonal carcinoma cells.
25233993 - Mrna-based therapeutics - developing a new class of drugs.
25263743 - Tpx2 as a novel prognostic biomarker for hepatocellular carcinoma.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-23
Journal Detail:
Title:  General and comparative endocrinology     Volume:  -     ISSN:  1095-6840     ISO Abbreviation:  Gen. Comp. Endocrinol.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370735     Medline TA:  Gen Comp Endocrinol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Inc.
Affiliation:
Department of Animal Physiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Live-cell subcellular measurement of cell stiffness using a microengineered stretchable micropost ar...
Next Document:  Ghrelin-induced growth hormone release from goldfish pituitary cells is nitric oxide dependent.