Document Detail

Pilot studies for proficiency testing using fluorescence in situ hybridization with chromosome-specific DNA probes: a College of American Pathologists/American College of Medical Genetics Program.
MedLine Citation:
PMID:  9140304     Owner:  NLM     Status:  MEDLINE    
Fluorescence in situ hybridization using chromosome-specific DNA probes is rapidly becoming part of clinical laboratory practice for certain congenital and neoplastic disorders. Current legislation requires proficiency testing for clinical laboratory studies. To evaluate the efficacy of fluorescence in situ hybridization proficiency testing, we invited 19 representative institutions to participate in three pilot studies. One study used probes for the X and Y chromosomes to evaluate metaphase spreads and interphase nuclei. Another study used probes for bcr and abl to detect bcr/abl fusion in interphase nuclei in chronic myelogenous leukemia. The third study used a D22S75 probe to detect microdeletions in metaphase spreads from a patient with velocardiofacial syndrome. The results of these studies demonstrate that proficiency testing with fluorescence in situ hybridization is attainable using either metaphase or interphase preparations, and that either microscope slides or fixed cell pellets are suitable.
G W Dewald; A R Brothman; M G Butler; L D Cooley; S R Patil; I A Saikevych; N R Schneider
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Archives of pathology & laboratory medicine     Volume:  121     ISSN:  0003-9985     ISO Abbreviation:  Arch. Pathol. Lab. Med.     Publication Date:  1997 Apr 
Date Detail:
Created Date:  1997-05-29     Completed Date:  1997-05-29     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7607091     Medline TA:  Arch Pathol Lab Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  359-67     Citation Subset:  AIM; IM    
Division of Laboratory Genetics, Mayo Clinic, Rochester, Minn 55905, USA.
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MeSH Terms
Chromosome Mapping / methods*
DNA Probes / diagnostic use*
In Situ Hybridization, Fluorescence / methods*
Pathology, Clinical / methods,  standards
Pilot Projects
Quality Control
Reproducibility of Results
Sensitivity and Specificity
Societies, Medical
United States
Reg. No./Substance:
0/DNA Probes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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