Document Detail

Pilot field trial of the EG95 vaccine against ovine cystic echinococcosis in Rio Negro, Argentina: early impact and preliminary data.
MedLine Citation:
PMID:  23632258     Owner:  NLM     Status:  Publisher    
Cystic echinococcosis is endemic in the Rio Negro province of Argentina. After 30 years of control using praziquantel in dogs the transmission rate to humans and sheep has decreased significantly, however transmission persists. The objective of the study is to assess the impact of the inclusion of the EG95 vaccine for sheep in the control program, including analysis of the vaccine's operative feasibility in field conditions. The vaccine was applied in an area comprising four communities of native people including 79 farms with 3146 lambs and 311 dogs in total. Seventy one farms were designated as control areas where no vaccinations were undertaken while vaccinations of lambs undertaken on 91 farms. Lambs received two vaccinations with the EG95 vaccine followed by a single booster injection when the animals were 1-1.5 years of age. Farm locations were defined using GPS coordinates for the houses. Evidence for Echinococcus granulosus transmission was monitored by coproantigen ELISA on samples of dog faeces, by E. granulosus-specific PCR using soil samples, and anti-E. granulosus antibody assessments in sera from 2-4 teeth lambs, purgation of dogs to detect E. granulosus worms and necropsy on adult sheep. Before the vaccine was introduced, 26.2% of sheep with 2-4 teeth were positive using ELISA/WB, the prevalence decreased to 7.8% at the third year following use of the vaccine. Necropsy of animals older than 6 years (not vaccinated) showed that 66.1% of animals were infected with E. granulosus. In dogs, 4% was found positive for E. granulosus using arecoline purgation and 24.7% of the farms were infected using coproELISA/WB. During the first year of vaccination 2721 lambs received the first vaccine dose and 2448 received a booster. In the second year 2138 lambs were initially vaccinated and 1745 received a booster, and 1308 animals received the third dose. During the third year 1110 lambs received the first dose from which 539 received a booster and 723 animals received the third dose. An analysis of advantages and limitations of the diagnostic techniques used and the ability of the geospatial analysis to detect risk area are included. Based in the immunodiagnostic techniques, the EG95 vaccine has been able to prevent the infection in animals up to 3 years old. Also, the difficulties in the field for the correct vaccine administration and the social features and habits that may impact on echinococcosis control are included in the analysis.
Edmundo Larrieu; Eduardo Herrero; Guillermo Mujica; Jose Luis Labanchi; Daniel Araya; Claudia Grizmado; Arnoldo Calabro; Gabriel Talmon; Guillermo Ruesta; Alicia Perez; Antonio Gatti; Graciela Santillán; Marta Cabrera; Marcos Arezzo; Marcos Seleiman; Laura Cavagión; Mariela García Cachau; Cristian A Alvarez Rojas; Lilia Gino; Charles G Gauci; David D Heath; Roberto Lamberti; Marshall W Lightowlers
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-4-27
Journal Detail:
Title:  Acta tropica     Volume:  -     ISSN:  1873-6254     ISO Abbreviation:  Acta Trop.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-5-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370374     Medline TA:  Acta Trop     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013. Published by Elsevier B.V.
Ministerio de Salud, Provincia de Río Negro, Argentina; Facultad de Ciencias Veterinarias, Universidad Nacional de La Pampa, Argentina. Electronic address:
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