Document Detail


Pilot study on "pericytic mimicry" and potential embryonic/stem cell properties of angiotropic melanoma cells interacting with the abluminal vascular surface.
MedLine Citation:
PMID:  23275074     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
The interaction of tumor cells with the tumor vasculature is mainly studied for its role in tumor angiogenesis and intravascular metastasis of circulating tumor cells. In addition, a specific interaction of tumor cells with the abluminal surfaces of vessels, or angiotropism, may promote the migration of angiotropic tumor cells along the abluminal vascular surfaces in a pericytic location. This process has been termed extravascular migratory metastasis. The abluminal vascular surface may also provide a vascular niche inducing or sustaining stemness to angiotropic tumor cells. This pilot study investigated if angiotropic melanoma cells might represent a subset population with pericytic and embryonic or stem cell properties. Through microarray analysis, we showed that the interaction between melanoma cells and the abluminal surface of endothelial cells triggers significant differential expression of several genes. The most significantly differentially expressed genes have demonstrated properties linked to cancer cell migration (CCL2, ICAM1 and IL6), cancer progression (CCL2, ICAM1, SELE, TRAF1, IL6, SERPINB2 and CXCL6), epithelial to mesenchymal transition (CCL2 and IL6), embryonic/stem cell properties (CCL2, PDGFB, EVX1 and CFDP1) and pericytic recruitment (PDGFB). In addition, bioinformatics-based analysis of the differentially expressed genes has shown that the most significantly enriched functional groups included development, cell movement, cancer, and embryonic development. Finally, the investigation of pericyte/mesenchymal stem cells markers via immunostaining of human melanoma samples revealed expression of PDGFRB, NG2 and CD146 by angiotropic melanoma cells. Taken together, these preliminary data are supportive of the "pericytic mimicry" by angiotropic melanoma cells, and suggest that the interaction between melanoma cells and the abluminal vascular surface induce differential expression of genes linked to cancer migration and embryonic/stem cell properties.
Authors:
Claire Lugassy; Madhuri Wadehra; Xinmin Li; Mirko Corselli; David Akhavan; Scott W Binder; Bruno Péault; Alistair J Cochran; Paul S Mischel; Hynda K Kleinman; Raymond L Barnhill
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Publication Detail:
Type:  Journal Article     Date:  2012-12-29
Journal Detail:
Title:  Cancer microenvironment : official journal of the International Cancer Microenvironment Society     Volume:  6     ISSN:  1875-2292     ISO Abbreviation:  Cancer Microenviron     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-19     Completed Date:  2013-03-20     Revised Date:  2014-08-20    
Medline Journal Info:
Nlm Unique ID:  101322634     Medline TA:  Cancer Microenviron     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  19-29     Citation Subset:  -    
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Grant Support
ID/Acronym/Agency:
G1000816//Medical Research Council; P30 CA016042/CA/NCI NIH HHS; T32 GM008042/GM/NIGMS NIH HHS
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