Document Detail


Pilot studies for development of an HIV subtype panel for surveillance of global diversity.
MedLine Citation:
PMID:  22149143     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The continued global spread and evolution of HIV diversity pose significant challenges to diagnostics and vaccine strategies. NIAID partnered with the FDA, WRAIR, academia, and industry to form a Viral Panel Working Group to design and prepare a panel of well-characterized current and diverse HIV isolates. Plasma samples that had screened positive for HIV infection and had evidence of recently acquired infection were donated by blood centers in North and South America, Europe, and Africa. A total of 80 plasma samples were tested by quantitative nucleic acid tests, p24 antigen, EIA, and Western blot to assign a Fiebig stage indicative of approximate time from initial infection. Evaluation of viral load using FDA-cleared assays showed excellent concordance when subtype B virus was tested, but lower correlations for subtype C. Plasma samples were cocultivated with phytohemagglutinin (PHA)-stimulated peripheral blood mononuclear cells (PBMCs) from normal donors to generate 30 viral isolates (50-80% success rate for samples with viral load >10,000 copies/ml), which were then expanded to 10(7)-10(9) virus copies per ml. Analysis of env sequences showed that sequences derived from cultured PBMCs were not distinguishable from those obtained from the original plasma. The pilot collection includes 30 isolates representing subtypes B, C, B/F, CRF04_cpx, and CRF02_AG. These studies will serve as a basis for the development of a comprehensive panel of highly characterized viral isolates that reflects the current dynamic and complex HIV epidemic, and will be made available through the External Quality Assurance Program Oversight Laboratory (EQAPOL).
Authors:
Mark Manak; Silvana Sina; Bharathi Anekella; Indira Hewlett; Eric Sanders-Buell; Viswanath Ragupathy; Jerome Kim; Marion Vermeulen; Susan L Stramer; Ester Sabino; Piotr Grabarczyk; Nelson Michael; Sheila Peel; Patricia Garrett; Sodsai Tovanabutra; Michael P Busch; Marco Schito
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Publication Detail:
Type:  Journal Article; Multicenter Study; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-02-24
Journal Detail:
Title:  AIDS research and human retroviruses     Volume:  28     ISSN:  1931-8405     ISO Abbreviation:  AIDS Res. Hum. Retroviruses     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-06-04     Completed Date:  2012-08-20     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  8709376     Medline TA:  AIDS Res Hum Retroviruses     Country:  United States    
Other Details:
Languages:  eng     Pagination:  594-606     Citation Subset:  IM; X    
Affiliation:
SeraCare Life Sciences, Inc., Gaithersburg, Maryland, USA. mmanak@hivresearch.org
Data Bank Information
Bank Name/Acc. No.:
GENBANK/JN687677;  JN687678;  JN687679;  JN687680;  JN687681;  JN687682;  JN687683;  JN687684;  JN687685;  JN687686;  JN687687;  JN687688;  JN687689;  JN687690;  JN687691;  JN687692;  JN687693;  JN687694;  JN687695;  JN687696;  JN687697;  JN687698;  JN687699;  JN687700;  JN687701;  JN687702;  JN687703;  JN687704;  JN687705;  JN687706;  JN687707;  JN687708;  JN687709;  JN687710;  JN687711;  JN687712;  JN687713;  JN687714;  JN687715;  JN687716;  JN687717;  JN687718;  JN687719;  JN687720;  JN687721;  JN687722;  JN687723;  JN687724;  JN687725;  JN687726;  JN687727;  JN687728;  JN687729;  JN687730;  JN687731;  JN687732;  JN687733;  JN687734;  JN687735;  JN687736;  JN687737;  JN687738;  JN687739;  JN687740;  JN687741;  JN687742;  JN687743;  JN687744;  JN687745;  JN687746;  JN687747;  JN687748;  JN687749;  JN687750;  JN687751;  JN687752;  JN687753;  JN687754;  JN687755;  JN687756;  JN687757;  JN687758;  JN687759;  JN687760;  JN687761;  JN687762;  JN687763;  JN687764;  JN687765;  JN687766;  JN687767;  JN687768;  JN687769;  JN687770;  JN687771;  JN687772;  JN687773;  JN687774;  JN687775;  JN687776;  JN687777;  JN687778;  JN687779;  JN687780;  JN687781;  JN687782;  JN687783;  JN687784;  JN687785;  JN687786;  JN687787;  JN687788;  JN687789;  JN687790;  JN687791;  JN687792;  JN687793;  JN687794;  JN687795;  JN687796;  JN687797;  JN687798;  JN687799;  JN687800;  JN687801;  JN687802;  JN687803;  JN687804;  JN687805;  JN687806;  JN687807;  JN687808;  JN687809;  JN687810;  JN687811;  JN687812;  JN687813;  JN687814;  JN687815;  JN687816;  JN687817;  JN687818;  JN687819;  JN687820;  JN687821;  JN687822;  JN687823;  JN687824;  JN687825;  JN687826;  JN687827;  JN687828;  JN687829;  JN687830;  JN687831;  JN687832;  JN687833;  JN687834;  JN687835;  JN687836;  JN687837;  JN687838;  JN687839;  JN687840;  JN687841;  JN687842;  JN687843;  JN687844;  JN687845;  JN687846;  JN687847
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MeSH Terms
Descriptor/Qualifier:
AIDS Vaccines / immunology*
Algorithms
Brazil / epidemiology
Cells, Cultured
Genome, Viral / immunology*
HIV Antibodies / immunology*
HIV Seropositivity / epidemiology,  genetics,  immunology*
HIV-1 / immunology*,  isolation & purification
Humans
Molecular Sequence Data
Phylogeny
Pilot Projects
Poland / epidemiology
Population Surveillance*
South Africa / epidemiology
United States / epidemiology
Grant Support
ID/Acronym/Agency:
HHSN272200800014C//PHS HHS
Chemical
Reg. No./Substance:
0/AIDS Vaccines; 0/HIV Antibodies
Comments/Corrections

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