Document Detail


PilT2 enhances the speed of gonococcal type IV pilus retraction and of twitching motility.
MedLine Citation:
PMID:  23035839     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Type IV pilus (T4P) dynamics is important for various bacterial functions including host cell interaction, surface motility, and horizontal gene transfer. T4P retract rapidly by depolymerization, generating large mechanical force. The gene that encodes the pilus retraction ATPase PilT has multiple paralogues, whose number varies between different bacterial species, but their role in regulating physical parameters of T4P dynamics remains unclear. Here, we address this question in the human pathogen Neisseria gonorrhoeae, which possesses two pilT paralogues, namely pilT2 and pilU. We show that the speed of twitching motility is strongly reduced in a pilT2 deletion mutant, while directional persistence time and sensitivity of speed to oxygen are unaffected. Using laser tweezers, we found that the speed of single T4P retraction was reduced by a factor of ≈ 2 in a pilT2 deletion strain, whereas pilU deletion showed a minor effect. The maximum force and the probability for switching from retraction to elongation under application of high force were not significantly affected. We conclude that the physical parameters of T4P are fine-tuned through PilT2.
Authors:
Rainer Kurre; Andrea Höne; Martin Clausen; Claudia Meel; Berenike Maier
Related Documents :
24463139 - Dkk2/frzb in the dermal papillae regulates feather regeneration.
23245319 - Nuclear repositioning precedes promoter accessibility and is linked to the switching fr...
23840039 - Copy-number variation of functional galectin genes: studying animal galectin-7 (p53-ind...
23155029 - Microarray analysis revealed that immunity-associated genes are primarily regulated by ...
24415769 - Transcriptional regulation of fruit ripening by tomato fruitfull homologs and associate...
16086309 - Worldwide haplotype diversity and coding sequence variation at human bitter taste recep...
9070329 - The expression of the mouse zic1, zic2, and zic3 gene suggests an essential role for zi...
21451579 - Explorations of high-intensity therapeutic ultrasound and microbubble-mediated gene del...
11053629 - A single nucleotide polymorphism in the first intron of the human ifn-gamma gene: absol...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-5
Journal Detail:
Title:  Molecular microbiology     Volume:  -     ISSN:  1365-2958     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 Blackwell Publishing Ltd.
Affiliation:
Department of Physics and Biocenter, University of Cologne, Zülpicher Str. 77, 50937, Köln, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Synthesis of Tubuphenylalanines via Ireland-Claisen Rearrangement.
Next Document:  Factors associated with successful decrease and discontinuation of antegrade continence enemas (ACE)...