| Pig transgenesis by Sleeping Beauty DNA transposition. | |
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MedLine Citation:
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PMID: 20803249 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Modelling of human disease in genetically engineered pigs provides unique possibilities in biomedical research and in studies of disease intervention. Establishment of methodologies that allow efficient gene insertion by non-viral gene carriers is an important step towards development of new disease models. In this report, we present transgenic pigs created by Sleeping Beauty DNA transposition in primary porcine fibroblasts in combination with somatic cell nuclear transfer by handmade cloning. Göttingen minipigs expressing green fluorescent protein are produced by transgenesis with DNA transposon vectors carrying the transgene driven by the human ubiquitin C promoter. These animals carry multiple copies (from 8 to 13) of the transgene and show systemic transgene expression. Transgene-expressing pigs carry both transposase-catalyzed insertions and at least one copy of randomly inserted plasmid DNA. Our findings illustrate critical issues related to DNA transposon-directed transgenesis, including coincidental plasmid insertion and relatively low Sleeping Beauty transposition activity in porcine fibroblasts, but also provide a platform for future development of porcine disease models using the Sleeping Beauty gene insertion technology. |
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Authors:
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Jannik E Jakobsen; Juan Li; Peter M Kragh; Brian Moldt; Lin Lin; Ying Liu; Mette Schmidt; Kjeld Dahl Winther; Brian Dall Schyth; Ida E Holm; Gábor Vajta; Lars Bolund; Henrik Callesen; Arne Lund Jørgensen; Anders Lade Nielsen; Jacob Giehm Mikkelsen |
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Publication Detail:
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Type: Journal Article Date: 2010-08-29 |
Journal Detail:
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Title: Transgenic research Volume: 20 ISSN: 1573-9368 ISO Abbreviation: Transgenic Res. Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-05-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9209120 Medline TA: Transgenic Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 533-45 Citation Subset: IM |
Affiliation:
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Department of Human Genetics, University of Aarhus, Wilh. Meyers Allé 1240, 8000, Aarhus C, Denmark. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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