Document Detail


Pig brain aldose reductase: purification, significance of the amino acid composition, substrate specificity and mechanism.
MedLine Citation:
PMID:  3121406     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. Pig brain aldose reductase (ALR2, EC 1.1.1.21) has been purified from fresh tissue with a approximately 60% improvement in specific activity over an acetone-powder preparation. 2. Dead-end inhibition and alternate substrate studies rule out an iso Theorell-Chance mechanism but are compatible with an ordered bi bi mechanism where NADPH and NADP+ function as the outside reactants in the direction of xylitol formation. 3. Subtle but significant differences are shown to exist in the distribution of apolar and mixed amino acid residues between aldose and aldehyde reductases when the mean fractional area loss [Rose et al., 1985] is used as the measure of compositional relatedness.
Authors:
H Yoo; E T McGuinness
Related Documents :
7537776 - Molecular analysis of elastic properties of the stratum corneum by solid-state 13c-nucl...
6842306 - Chronic suppression of cholesterol 7 alpha-hydroxylase by dietary chenodeoxycholic acid...
9744906 - The sensitizing capacity of ginkgolic acids in guinea pigs.
3977766 - Dissociation of the cochlear microphonics and endocochlear potential after injection of...
23196006 - The composition of the lipid constituents of ilex aquifolium l. {aquifoliaceae) in rela...
8900016 - Epidemiological typing of bacillus spp. isolated from food.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The International journal of biochemistry     Volume:  19     ISSN:  0020-711X     ISO Abbreviation:  Int. J. Biochem.     Publication Date:  1987  
Date Detail:
Created Date:  1988-02-12     Completed Date:  1988-02-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0250365     Medline TA:  Int J Biochem     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  865-71     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Seton Hall University, South Orange, NJ 07079.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Aldehyde Reductase / isolation & purification*,  metabolism
Amino Acids / analysis
Animals
Brain / enzymology*
Chromatography, Affinity
Chromatography, Ion Exchange
Kinetics
Substrate Specificity
Sugar Alcohol Dehydrogenases / isolation & purification*
Swine
Chemical
Reg. No./Substance:
0/Amino Acids; EC 1.1.-/Sugar Alcohol Dehydrogenases; EC 1.1.1.21/Aldehyde Reductase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Chain-fluorinated polyamines as tumor markers--I. In vivo transformation of 2,2-difluoroputrescine i...
Next Document:  Estradiol positive feedback on the rat anterior pituitary gland in vitro.