Document Detail


Piezo-assisted in vitro fertilization of mouse oocytes with spermatozoa retrieved from epididymides stored at 4 degree C.
MedLine Citation:
PMID:  18239354     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study was performed to investigate the effect of partial zona pellucida incision by piezo micromanipulation (ZIP) on the in vitro fertilizing ability of stored mouse spermatozoa. The storage conditions were optimized by storing the mouse epididymides at 4 C in mineral oil or in the mouse body for up to 4 days after death, and the retrieved spermatozoa were used to fertilize fresh oocytes. No significant difference was observed in fertilization rates between the treatments when epididymides were stored for up to 2 days, but the fertilization rates in mineral oil were higher (P<0.05) than those in the mouse body at 3 (41.4 vs. 16.2%) and 4 days (26.0 vs. 15.8%). Spermatozoa retrieved from epididymides stored in mineral oil were then used to fertilize fresh and vitrified oocytes with or without ZIP treatment. The fertilization rates of the ZIP fresh oocytes were higher than those of the zona-intact oocytes at each time point (1 to 4 days). After ZIP, the fertilization rates of spermatozoa stored for 1 and 2 days (91.2 and 86.6%, respectively) were similar (P>0.05) to that of fresh spermatozoa (91.9%). In regard to vitrified oocytes, the fertilization rates of zona-intact and ZIP oocytes using fresh spermatozoa were 46.7 and 84.7%, while the fertilization rates of vitrified ZIP oocytes using spermatozoa stored for 1 to 4 days ranged from 49.3 to 79.6%. When 2-cell embryos derived from ZIP fresh and vitrified oocytes inseminated with 2 day-stored spermatozoa were transferred into recipient females, 47.9 and 15.0% of the embryos developed to term, respectively. These results indicate that storing mouse epididymides at 4 C in mineral oil is more suitable than storage in the mouse body and that the ZIP technique improves the in vitro fertilizing ability of stored mouse spermatozoa in fresh oocytes and significantly increases the fertilization rate of vitrified oocytes with fresh spermatozoa.
Authors:
Zhi-Qiang Fan; Xiu-Wei Li; Ying Liu; Qing-Gang Meng; Yan-Ping Wang; Yun-Peng Hou; Guang-Bin Zhou; Shi-En Zhu
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Publication Detail:
Type:  Journal Article     Date:  2008-01-30
Journal Detail:
Title:  The Journal of reproduction and development     Volume:  54     ISSN:  0916-8818     ISO Abbreviation:  J. Reprod. Dev.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-04-30     Completed Date:  2008-07-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9438792     Medline TA:  J Reprod Dev     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  107-12     Citation Subset:  IM    
Affiliation:
Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, Beijing, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Embryo Transfer
Epididymis / metabolism*
Female
Fertilization in Vitro / methods*
Male
Mice
Mineral Oil / metabolism
Oocytes / metabolism*
Pregnancy
Reproductive Techniques, Assisted*
Semen Preservation / methods
Specimen Handling
Spermatozoa / metabolism*
Temperature
Chemical
Reg. No./Substance:
8020-83-5/Mineral Oil

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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