Document Detail


Phytosterols in physiological concentrations target multidrug resistant cancer cells.
MedLine Citation:
PMID:  20843282     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Phytosterols have been proposed to act as potent anticancer agents. However the mechanism of their action has not been elucidated yet. Thus, the aim of our study was to determine whether plant sterols and their thermal processing products (in physiological concentration range) could influence the viability of cancer cells and thus could be considered as positive diet complements. Additionally we decided to study potential specificity of those natural compounds against cells showing high multidrug resistance. In this study we show that the cytotoxic effect of β-sitosterol was observed in both, estrogen-dependent and estrogen-independent cells. It was also shown that the β-sitosterol was significantly more cytotoxic in cells with basal ABCB1 expression (MCF7) than in multidrug resistant NCI/ADR-RES. Surprisingly, 5a,6a-epoxysitosterol did not decrease the viability of any investigated cells but on the contrary, it provoked their increased proliferation. It was shown that oxyphytosterols blocked the cell cycle of MCF7 cells in G0/G1 phase while did not affect NCI/ADR-RES cell cycle in physiological concentration range. We also show that PgP activity (responsible for Multidrug Resistance phenomena) is inhibited by β-sitosterol. Thus, the phytosterols are supposed to act at various mechanisms but, what is most interesting, can target cells showing high multidrug resistance potential.
Authors:
Blazej Rubis; Anna Polrolniczak; Hanna Knula; Olga Potapinska; Mariusz Kaczmarek; Maria Rybczynska
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Medicinal chemistry (Shāriqah (United Arab Emirates))     Volume:  6     ISSN:  1875-6638     ISO Abbreviation:  Med Chem     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-10-14     Completed Date:  2011-02-16     Revised Date:  2012-03-14    
Medline Journal Info:
Nlm Unique ID:  101240303     Medline TA:  Med Chem     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  184-90     Citation Subset:  IM    
Affiliation:
Poznan University of Medical Sciences, Department of Clinical Chemistry and Molecular Diagnostics, Przybyszewskiego 49, 60-355 Poznan, Poland. blazejr@ump.edu.pl
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents / pharmacology*
Breast Neoplasms / drug therapy*,  metabolism,  pathology*
Cell Cycle / drug effects
Cell Survival / drug effects
Dose-Response Relationship, Drug
Drug Resistance, Multiple / drug effects*
Drug Resistance, Neoplasm / drug effects*
Humans
P-Glycoprotein / antagonists & inhibitors,  metabolism
Phytosterols / pharmacology*
Structure-Activity Relationship
Tumor Cells, Cultured
Verapamil / pharmacology
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/P-Glycoprotein; 0/Phytosterols; 52-53-9/Verapamil

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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