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Phytosterol ester processing in the small intestine; impact on cholesterol availability for absorption and chylomicron cholesterol incorporation in healthy humans.
MedLine Citation:
PMID:  21482714     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Phytosterols (plant sterols and stanols) can lower intestinal cholesterol (C) absorption, but the complex dynamics of the lipid digestion process in the presence of plant sterol esters (PE) are not fully understood. We performed a clinical experiment in intubated healthy subjects to study the time course of changes in the distribution of all lipid moieties present in duodenal phases during 4 h of digestion of meals with 3.2 g PE (PE meal) or without (control meal) PE. In vitro experiments under simulated gastro-intestinal conditions were also performed. The addition of PE did not alter triglyceride hydrolysis in the duodenum or subsequent chylomicron triglyceride occurrence in the circulation. In contrast, C accumulation in the duodenum aqueous phase was markedly reduced in the presence of PE (-32%, p<0.10). In vitro experiments confirmed that PE reduces C transfer into the aqueous phase. The addition of PE resulted in a markedly reduced presence of meal-derived hepta-deuterated cholesterol (D7C) in the circulation, i.e., in chylomicrons (-43%, PE-meal vs. control; p<0.0001) and plasma (-54%, PE meal vs. control; p<0.0001). The present data show that addition of PE into a meal does not alter TG hydrolysis but displace C from the intestinal aqueous phase and lower chylomicron C occurrence in humans.
Authors:
Marie Josephe Amiot; Diny Knol; Nicolas Cardinault; Marion Nowicki; Romain Bott; Claudine Antona; Patrick Borel; Jean-Paul Bernard; Guus Duchateau; Denis Lairon
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-4-11
Journal Detail:
Title:  Journal of lipid research     Volume:  -     ISSN:  0022-2275     ISO Abbreviation:  -     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-4-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
INRA, France;
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