Document Detail


Phytosterol-deficient and high-phytosterol diets developed for controlled feeding studies.
MedLine Citation:
PMID:  19942022     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Phytosterols reduce cholesterol absorption and low-density lipoprotein cholesterol concentrations, but the quantity and physiological significance of phytosterols in common diets are generally unknown because nutrient databases do not contain comprehensive phytosterol data. The primary aim of this study was to design prototype phytosterol-deficient and high-phytosterol diets for use in controlled feeding studies of the influence of phytosterols on health. A second aim was to quantify the phytosterol content of these prototype diets and three other diets consumed in the United States. This study was conducted from June 2001 to September 2008 and involved designing, preparing, and then analyzing five different diets: an experimental phytosterol-deficient control diet, a relatively high-phytosterol diet based on the Dietary Approaches to Stop Hypertension diet, American Heart Association diet, Atkins lifetime maintenance plan, and a vegan diet. A single day of meals for each diet was homogenized and the resulting composites were analyzed for free, esterified, and glycosylated phytosterols by gas chromatography. Independent samples t tests were used to compare the diets' total phytosterol content. The total phytosterol content of the experimental phytosterol-deficient diet was 64 mg/2,000 kcal, with progressively larger quantities in Atkins, American Heart Association, vegan, and the high-phytosterol Dietary Approaches to Stop Hypertension diet (163, 340, 445, and 500 mg/2,000 kcal, respectively). Glycosylated phytosterols, which are often excluded from phytosterol analyses, comprised 15.9%+/-5.9% of total phytosterols. In summary, phytosterol-deficient and high-phytosterol diets that conform to recommended macronutrient guidelines and are palatable can now be used in controlled feeding studies.
Authors:
Susan B Racette; Catherine Anderson Spearie; Katherine M Phillips; Xiaobo Lin; Lina Ma; Richard E Ostlund
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of the American Dietetic Association     Volume:  109     ISSN:  1878-3570     ISO Abbreviation:  J Am Diet Assoc     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-11-27     Completed Date:  2009-12-15     Revised Date:  2011-03-03    
Medline Journal Info:
Nlm Unique ID:  7503061     Medline TA:  J Am Diet Assoc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2043-51     Citation Subset:  AIM; IM    
Affiliation:
Department of Medicine and Program in Physical Therapy, Washington University School of Medicine, Campus Box 8502, 4444 Forest Park Ave, St Louis, MO 63108, USA. racettes@wustl.edu
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MeSH Terms
Descriptor/Qualifier:
Cholesterol, Dietary / metabolism,  pharmacokinetics*
Chromatography, Gas
Diet, Carbohydrate-Restricted
Diet, Sodium-Restricted
Diet, Vegetarian
Dose-Response Relationship, Drug
Food Analysis*
Humans
Hypercholesterolemia / diet therapy*
Patient Satisfaction
Phytosterols / administration & dosage,  analysis*,  pharmacology*
Taste
Grant Support
ID/Acronym/Agency:
M01 RR000036-44/RR/NCRR NIH HHS; M01 RR000036-45/RR/NCRR NIH HHS; M01 RR000036-46/RR/NCRR NIH HHS; M01 RR000036-47/RR/NCRR NIH HHS; MO1 RR-00036/RR/NCRR NIH HHS; P30 DK056341/DK/NIDDK NIH HHS; P30 DK056341-08/DK/NIDDK NIH HHS; P30 DK056341-09/DK/NIDDK NIH HHS; P41 RR000954-27S1/RR/NCRR NIH HHS; R01 HL050420-07/HL/NHLBI NIH HHS; R01 HL050420-12/HL/NHLBI NIH HHS; RR-00954/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Cholesterol, Dietary; 0/Phytosterols

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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