Document Detail


Phytohemagglutinin induces rapid degradation of phosphatidylinositol 4,5-bisphosphate and transient accumulation of phosphatidic acid and diacylglycerol in a human T lymphoblastoid cell line, CCRF-CEM.
MedLine Citation:
PMID:  6317043     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The human T lymphoblastoid cell line designated CCRF-CEM responds to phytohemagglutinin with a 3.7-fold enhancement of the 32PO4 incorporation into phosphatidylinositol. In myo-[2-3H]inositol-prelabeled CCRF-CEM cells, phytohemagglutinin induced a 3.3-fold accumulation of myo-[2-3H]inositol phosphate during 15 min incubation at 37 degrees C in the presence of 5 mM LiCl. Since Li+ is a potent inhibitor of myo-inositol-1-phosphatase, the results indicate that phytohemagglutinin induces the hydrolysis of inositol lipids in CCRF-CEM cells. In 32PO4-prelabeled CCRF-CEM cells, phytohemagglutinin induced a breakdown of 28% of [32P]phosphatidylinositol 4,5-bisphosphate 40-60 s after the stimulation. The decrease of [32P]phosphatidylinositol 4,5-bisphosphate was found as early as 10 s after the stimulation. This decrease was followed by an increased 32P-labeling of phosphatidic acid. In [2-3H]glycerol-prelabeled CCRF-CEM cells, phytohemagglutinin induced a transient accumulation of [3H]phosphatidic acid and [3H]diacylglycerol. The amount of [3H]phosphatidic acid in the stimulated cells was 3.7-times the control value at 2 min after the stimulation, whereas the amount of [3H]diacylglycerol in the stimulated cells was 1.5-times the control value at 5 min after the stimulation. In [3H8]arachidonate-prelabeled CCRF-CEM cells, phytohemagglutinin induced a transient accumulation of [3H]phosphatidic acid; the amount was 2.5-times the control value at 2 min after the stimulation. Quinacrine (1 mM) caused 41% reduction in the amount of [3H]phosphatidic acid accumulated by the stimulation in [2-3H]glycerol-prelabeled cells. Stimulation in a Ca2+-free saline containing 1 mM EGTA caused 53% reduction in the amount of [3H]phosphatidic acid accumulated by the stimulation. The results presented in this paper indicate that a human T lymphoblastoid cell line, CCRF-CEM, responds to phytohemagglutinin with a rapid turnover of inositol lipids.
Authors:
H Hasegawa-Sasaki; T Sasaki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  754     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1983 Dec 
Date Detail:
Created Date:  1984-02-15     Completed Date:  1984-02-15     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  305-14     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Line
Concanavalin A / pharmacology*
Diglycerides / metabolism*
Glycerides / metabolism*
Humans
Kinetics
Leukemia, Lymphoid / metabolism*
Phosphatidic Acids / metabolism*
Phosphatidylinositol 4,5-Diphosphate
Phosphatidylinositols / metabolism*
Phospholipids / biosynthesis
Phosphorus Radioisotopes / diagnostic use
Phytohemagglutinins / pharmacology
T-Lymphocytes / metabolism
Chemical
Reg. No./Substance:
0/Diglycerides; 0/Glycerides; 0/Phosphatidic Acids; 0/Phosphatidylinositol 4,5-Diphosphate; 0/Phosphatidylinositols; 0/Phospholipids; 0/Phosphorus Radioisotopes; 0/Phytohemagglutinins; 0/phytohemagglutinin-P; 11028-71-0/Concanavalin A

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