| Phytoferritin is synthesized in vitro as a high-molecular-weight precursor. Studies on the synthesis and the uptake in vitro of the precursors of ferritin and ferredoxin by intact chloroplasts. | |
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MedLine Citation:
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PMID: 6626165 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Evidence is presented that French-bean (Phaseolus vulgaris) seed ferritin is composed of one type of subunit with an apparent Mr of 26500. In normal and iron-loaded leaf tissues it is detected immunologically with an antiserum raised against purified bean seed ferritin and migrates in SDS (sodium dodecyl sulphate)/polyacrylamide-gel electrophoresis with the same mobility as the bean seed ferritin subunit. The biosynthetic pathway of ferritin in normal and iron-loaded leaves was investigated. RNA was extracted, fractionated into polyadenylated RNA and translated in a cell-free rabbit reticulocyte lysate and a wheat-germ-extract system. The products were identified by SDS/polyacrylamide-gel electrophoresis after indirect immunoprecipitation. In all cases the ferritin product had an Mr 5000 higher than that of the native subunit. Uptake and processing of the precursor form of ferritin from iron-loaded leaves by intact chloroplasts was demonstrated. This indicates that, in iron-loaded leaves, ferritin acts as a chloroplast protein. We propose that the ferritin precursor in normal leaves follows the same biosynthetic pathway. This suggests that the iron-buffering function of ferritin in plants takes place in the chloroplast and that non-functional cellular iron will accumulate in this cell organelle. |
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Authors:
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F van der Mark; W van den Briel; H G Huisman |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Biochemical journal Volume: 214 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1983 Sep |
Date Detail:
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Created Date: 1983-11-23 Completed Date: 1983-11-23 Revised Date: 2010-09-13 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 943-50 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Carrier Proteins
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immunology Chloroplasts / metabolism* Electrophoresis, Polyacrylamide Gel Fabaceae / metabolism Ferredoxins / biosynthesis* Ferritins / biosynthesis*, immunology Molecular Weight Plant Proteins / immunology Plants, Medicinal Protein Biosynthesis Seeds / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Ferredoxins; 0/Plant Proteins; 0/phytoferritin; 9007-73-2/Ferritins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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