Document Detail


Phytoferritin is synthesized in vitro as a high-molecular-weight precursor. Studies on the synthesis and the uptake in vitro of the precursors of ferritin and ferredoxin by intact chloroplasts.
MedLine Citation:
PMID:  6626165     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Evidence is presented that French-bean (Phaseolus vulgaris) seed ferritin is composed of one type of subunit with an apparent Mr of 26500. In normal and iron-loaded leaf tissues it is detected immunologically with an antiserum raised against purified bean seed ferritin and migrates in SDS (sodium dodecyl sulphate)/polyacrylamide-gel electrophoresis with the same mobility as the bean seed ferritin subunit. The biosynthetic pathway of ferritin in normal and iron-loaded leaves was investigated. RNA was extracted, fractionated into polyadenylated RNA and translated in a cell-free rabbit reticulocyte lysate and a wheat-germ-extract system. The products were identified by SDS/polyacrylamide-gel electrophoresis after indirect immunoprecipitation. In all cases the ferritin product had an Mr 5000 higher than that of the native subunit. Uptake and processing of the precursor form of ferritin from iron-loaded leaves by intact chloroplasts was demonstrated. This indicates that, in iron-loaded leaves, ferritin acts as a chloroplast protein. We propose that the ferritin precursor in normal leaves follows the same biosynthetic pathway. This suggests that the iron-buffering function of ferritin in plants takes place in the chloroplast and that non-functional cellular iron will accumulate in this cell organelle.
Authors:
F van der Mark; W van den Briel; H G Huisman
Related Documents :
10393315 - Hyperproduction of recombinant ferredoxins in escherichia coli by coexpression of the o...
20406405 - Atr3 encodes a diflavin reductase essential for arabidopsis embryo development.
17211635 - Purification, characterization, and crystallization of the components of a biphenyl dio...
12459205 - Molecular cloning, expression and isolation of ferritins from two tick species--ornitho...
23134305 - Prokaryotic expression and immunogenicity analysis of yak recombinant myostatin.
20154125 - Analysis of protein expression regulated by the helicobacter pylori arsrs two-component...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  214     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1983 Sep 
Date Detail:
Created Date:  1983-11-23     Completed Date:  1983-11-23     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  943-50     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Carrier Proteins / immunology
Chloroplasts / metabolism*
Electrophoresis, Polyacrylamide Gel
Fabaceae / metabolism
Ferredoxins / biosynthesis*
Ferritins / biosynthesis*,  immunology
Molecular Weight
Plant Proteins / immunology
Plants, Medicinal
Protein Biosynthesis
Seeds / metabolism
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Ferredoxins; 0/Plant Proteins; 0/phytoferritin; 9007-73-2/Ferritins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Development and regulation of ketogenesis in hepatocytes isolated from newborn rats.
Next Document:  A critical appraisal of the effect of oxidized glutathione on hepatic glucose 6-phosphate dehydrogen...