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Phytochemical composition and in vitro antioxidant activity of aqueous extract of Aerva lanata (L.) Juss. ex Schult. Stem (Amaranthaceae).
MedLine Citation:
PMID:  23375030     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
OBJECTIVE: To analyze the phytochemical composition and in vitro antioxidant properties of aqueous extract of Aerva lanata (A. lanata) stem.
METHODS: During the preliminary phytochemical analysis, the aqueous extract of A. lanata was screened for the presence of carbohydrates, proteins, phenolic compounds, oil and fats, saponins, flavonoids, alkaloids, tannins and phytosterols. Antioxidant activity of the extract was determined by 2, 2-diphenyl-1-picrylhydrazyl radical scavenging activity, metal chelating activity, reducing power activity and DNA damage inhibition activity. Analysis of phenolic compounds was performed by Folin-Ciocalteau reagent method and gradient high performance liquid chromatography technique.
RESULTS: Preliminary phytochemical analysis exhibited the presence of phenolic compounds, saponins, flavonoids, tannins and phytosterols as major phytochemical groups. The extract exhibited high 2, 2-diphenyl-1-picrylhydrazyl radical scavenging activity (IC(50)= 110.74 μg/mL), metal chelating activity (IC(50)= 758.17 μg/mL), reducing power activity and DNA damage inhibition efficiency. The extract was reported to possess a high amount of total phenolic content and some of them were identified as gallic acid (3,4,5-OH), apigenin-7-O-glucoside (apigetrin), quercetin-3-O-rutinoside (rutin) and myricetin (3,5,7,3,4,5-OH) by high performance liquid chromatography analysis. The extract was found non toxic towards human erythrocytes in the hemolytic assay (IC(50) = 24.89 mg/mL).
CONCLUSIONS: These results conclud that A. lanata stem possesses high antioxidant activity and can be used for the development of natural and safe antioxidant compounds.
Authors:
Gaurav Kumar; Loganathan Karthik; Kokati Vankata Bhaskara Rao
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Asian Pacific journal of tropical medicine     Volume:  6     ISSN:  1995-7645     ISO Abbreviation:  Asian Pac J Trop Med     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-04     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101533720     Medline TA:  Asian Pac J Trop Med     Country:  Singapore    
Other Details:
Languages:  eng     Pagination:  180-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Hainan Medical College. Published by Elsevier B.V. All rights reserved.
Affiliation:
Molecular and Microbiology Research Laboratory, Environmental Biotechnology Division, School of Bio Sciences and Technology, VIT University, Vellore, Tamil Nadu - 632 014, India.
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