Document Detail


Phytochemical characterization and radical scavenger activity of flavonoids from Helichrysum italicum G. Don (Compositae).
MedLine Citation:
PMID:  2075159     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The glycosidic fraction of the flavonoids extracted from the flowering tops of the Helichrysum italicum G. Don was isolated, purified and characterized. This fraction was constituted by three compounds, which were assigned the structure of 4,2',4',6'-tetrahydroxychalcone-2'-glucoside, kaempferol-3-glucoside and naringenin-glycoside. Radical scavenger properties of the single glycosyl-flavonoids and of the in toto glycosidic fraction were tested with in vitro systems where different reactive oxygen species are generated (superoxide ions, hydroxyl radicals) and on lipid peroxidation induced by ADP/Fe2+ and NADPH or CCl4 in rat liver microsomes. The formation of reactive oxygen species was detected by cytochrome c reduction, salicylic acid hydroxylation and hyaluronic acid depolymerization. The action of the glycosidic fraction on the release of TXB2 and 12-HETE in human platelets, after collagen stimulation, was also evaluated. The glycosidic fraction inhibited in a dose dependent fashion lipid peroxidation in rat liver microsomes treated with ADP/Fe2+ or CCl4. This effect is due to the ability of flavonoids to scavenge free radicals at different stages of the process (superoxide ions, hydroxyl and lipid peroxide radicals). The single glycosyl-flavonoids exhibited a different scavenger activity, depending on the oxygen species and the chemical structure of the compounds. No effect of the fraction was observed on TXB2 and 12-HETE formation at 100 microns concentration.
Authors:
R M Facino; M Carini; L Franzoi; O Pirola; E Bosisio
Related Documents :
8161499 - Free radical-mediated lipid peroxidation in cells: oxidizability is a function of cell ...
17250799 - Saliva plays a dual role in oxidation process in stomach medium.
2161619 - Generation of reactive oxygen species and reduction of ferric chelates by microsomes in...
11437179 - Inhibitory effects of echinoisoflavanone and sophoraisoflavanone d in sophora chrysophy...
15220329 - Supplementation of endothelial cells with mitochondria-targeted antioxidants inhibit pe...
2125819 - Phospholipid peroxidation induced by the catechol-fe3+(cu2+) complex: a possible mechan...
23465899 - Production methods for heparosan, a precursor of heparin and heparan sulfate.
16674089 - Efficient synthesis of 2'-c-beta-methylguanosine.
16348719 - Sequential transhydroxylations converting hydroxyhydroquinone to phloroglucinol in the ...
Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Pharmacological research : the official journal of the Italian Pharmacological Society     Volume:  22     ISSN:  1043-6618     ISO Abbreviation:  Pharmacol. Res.     Publication Date:    1990 Nov-Dec
Date Detail:
Created Date:  1991-04-12     Completed Date:  1991-04-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8907422     Medline TA:  Pharmacol Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  709-21     Citation Subset:  IM    
Affiliation:
Chemical Pharmaceutical Toxicological Institute, Faculty of Pharmacy, University of Milan, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
Animals
Blood Platelets / drug effects,  metabolism
Chromatography, Thin Layer
Female
Flavonoids / analysis,  pharmacology*
Free Radical Scavengers
Free Radicals
Glycosides / analysis
Humans
Hyaluronic Acid / analysis
Hydroxyeicosatetraenoic Acids / metabolism
Lipid Peroxides / metabolism
Magnetic Resonance Spectroscopy
Male
Microsomes, Liver / metabolism
NADH Dehydrogenase / metabolism
Plant Extracts / analysis,  pharmacology*
Rats
Rats, Inbred Strains
Solvents
Thromboxane B2 / metabolism
Chemical
Reg. No./Substance:
0/Flavonoids; 0/Free Radical Scavengers; 0/Free Radicals; 0/Glycosides; 0/Hydroxyeicosatetraenoic Acids; 0/Lipid Peroxides; 0/Plant Extracts; 0/Solvents; 54397-85-2/Thromboxane B2; 59985-28-3/12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; 9004-61-9/Hyaluronic Acid; EC 1.6.99.3/NADH Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Effect of anti-relapse antimalarial compound CDRI 80/53 and primaquine on hepatic mixed function oxi...
Next Document:  Historical perspectives of child psychiatric research in the tension field between natural and psych...