Document Detail

Phytochemical characterization and radical scavenger activity of flavonoids from Helichrysum italicum G. Don (Compositae).
MedLine Citation:
PMID:  2075159     Owner:  NLM     Status:  MEDLINE    
The glycosidic fraction of the flavonoids extracted from the flowering tops of the Helichrysum italicum G. Don was isolated, purified and characterized. This fraction was constituted by three compounds, which were assigned the structure of 4,2',4',6'-tetrahydroxychalcone-2'-glucoside, kaempferol-3-glucoside and naringenin-glycoside. Radical scavenger properties of the single glycosyl-flavonoids and of the in toto glycosidic fraction were tested with in vitro systems where different reactive oxygen species are generated (superoxide ions, hydroxyl radicals) and on lipid peroxidation induced by ADP/Fe2+ and NADPH or CCl4 in rat liver microsomes. The formation of reactive oxygen species was detected by cytochrome c reduction, salicylic acid hydroxylation and hyaluronic acid depolymerization. The action of the glycosidic fraction on the release of TXB2 and 12-HETE in human platelets, after collagen stimulation, was also evaluated. The glycosidic fraction inhibited in a dose dependent fashion lipid peroxidation in rat liver microsomes treated with ADP/Fe2+ or CCl4. This effect is due to the ability of flavonoids to scavenge free radicals at different stages of the process (superoxide ions, hydroxyl and lipid peroxide radicals). The single glycosyl-flavonoids exhibited a different scavenger activity, depending on the oxygen species and the chemical structure of the compounds. No effect of the fraction was observed on TXB2 and 12-HETE formation at 100 microns concentration.
R M Facino; M Carini; L Franzoi; O Pirola; E Bosisio
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Pharmacological research : the official journal of the Italian Pharmacological Society     Volume:  22     ISSN:  1043-6618     ISO Abbreviation:  Pharmacol. Res.     Publication Date:    1990 Nov-Dec
Date Detail:
Created Date:  1991-04-12     Completed Date:  1991-04-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8907422     Medline TA:  Pharmacol Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  709-21     Citation Subset:  IM    
Chemical Pharmaceutical Toxicological Institute, Faculty of Pharmacy, University of Milan, Italy.
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MeSH Terms
12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
Blood Platelets / drug effects,  metabolism
Chromatography, Thin Layer
Flavonoids / analysis,  pharmacology*
Free Radical Scavengers
Free Radicals
Glycosides / analysis
Hyaluronic Acid / analysis
Hydroxyeicosatetraenoic Acids / metabolism
Lipid Peroxides / metabolism
Magnetic Resonance Spectroscopy
Microsomes, Liver / metabolism
NADH Dehydrogenase / metabolism
Plant Extracts / analysis,  pharmacology*
Rats, Inbred Strains
Thromboxane B2 / metabolism
Reg. No./Substance:
0/Flavonoids; 0/Free Radical Scavengers; 0/Free Radicals; 0/Glycosides; 0/Hydroxyeicosatetraenoic Acids; 0/Lipid Peroxides; 0/Plant Extracts; 0/Solvents; 54397-85-2/Thromboxane B2; 59985-28-3/12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; 9004-61-9/Hyaluronic Acid; EC Dehydrogenase

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