| Phytanic acid, a novel activator of uncoupling protein-1 gene transcription and brown adipocyte differentiation. | |
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MedLine Citation:
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PMID: 11829740 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Phytanic acid (3,7,11,15-tetramethylhexadecanoic acid) is a phytol-derived branched-chain fatty acid present in dietary products. Phytanic acid increased uncoupling protein-1 (UCP1) mRNA expression in brown adipocytes differentiated in culture. Phytanic acid induced the expression of the UCP1 gene promoter, which was enhanced by co-transfection with a retinoid X receptor (RXR) expression vector but not with other expression vectors driving peroxisome proliferator-activated receptor (PPAR)alpha, PPARgamma or a form of RXR devoid of ligand-dependent sensitivity. The effect of phytanic acid on the UCP1 gene required the 5' enhancer region of the gene and the effects of phytanic acid were mediated in an additive manner by three binding sites for RXR. Moreover, phytanic acid activates brown adipocyte differentiation: long-term exposure of brown preadipocytes to phytanic acid promoted the acquisition of the brown adipocyte morphology and caused a co-ordinate induction of the mRNAs for gene markers of brown adipocyte differentiation, such as UCP1, adipocyte lipid-binding protein aP2, lipoprotein lipase, the glucose transporter GLUT4 or subunit II of cytochrome c oxidase. In conclusion, phytanic acid is a natural product of phytol metabolism that activates brown adipocyte thermogenic function. It constitutes a potential nutritional signal linking dietary status to adaptive thermogenesis. |
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Authors:
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Agatha Schlüter; Maria José Barberá; Roser Iglesias; Marta Giralt; Francesc Villarroya |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Biochemical journal Volume: 362 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 2002 Feb |
Date Detail:
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Created Date: 2002-02-06 Completed Date: 2002-03-22 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: England |
Other Details:
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Languages: eng Pagination: 61-9 Citation Subset: IM |
Affiliation:
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Departament de Bioquímica i Biologia Molecular, Universitat de Barcelona, Facultat de Biologia, Avda Diagonal 645, Barcelona 08028, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adipocytes
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cytology,
drug effects* Adipose Tissue, Brown / cytology, drug effects* Animals Carrier Proteins / genetics* Cell Differentiation / drug effects* Cells, Cultured Ion Channels Membrane Proteins / genetics* Mice Mitochondrial Proteins Phytanic Acid / pharmacology* Promoter Regions, Genetic Protein Binding Receptors, Retinoic Acid / metabolism Retinoid X Receptors Transcription Factors / metabolism Transcriptional Activation / drug effects* |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Ion Channels; 0/Membrane Proteins; 0/Mitochondrial Proteins; 0/Receptors, Retinoic Acid; 0/Retinoid X Receptors; 0/Transcription Factors; 0/mitochondrial uncoupling protein; 14721-66-5/Phytanic Acid |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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