Document Detail


Physiology of consumption of human milk oligosaccharides by infant gut-associated bifidobacteria.
MedLine Citation:
PMID:  21832085     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The bifidogenic effect of human milk oligosaccharides (HMOs) has long been known, yet the precise mechanism underlying it remains unresolved. Recent studies show that some species/subspecies of Bifidobacterium are equipped with genetic and enzymatic sets dedicated to the utilization of HMOs, and consequently they can grow on HMOs; however, the ability to metabolize HMOs has not been directly linked to the actual metabolic behavior of the bacteria. In this report, we clarify the fate of each HMO during cultivation of infant gut-associated bifidobacteria. Bifidobacterium bifidum JCM1254, Bifidobacterium longum subsp. infantis JCM1222, Bifidobacterium longum subsp. longum JCM1217, and Bifidobacterium breve JCM1192 were selected for this purpose and were grown on HMO media containing a main neutral oligosaccharide fraction. The mono- and oligosaccharides in the spent media were labeled with 2-anthranilic acid, and their concentrations were determined at various incubation times using normal phase high performance liquid chromatography. The results reflect the metabolic abilities of the respective bifidobacteria. B. bifidum used secretory glycosidases to degrade HMOs, whereas B. longum subsp. infantis assimilated all HMOs by incorporating them in their intact forms. B. longum subsp. longum and B. breve consumed lacto-N-tetraose only. Interestingly, B. bifidum left degraded HMO metabolites outside of the cell even when the cells initiate vegetative growth, which indicates that the different species/subspecies can share the produced sugars. The predominance of type 1 chains in HMOs and the preferential use of type 1 HMO by infant gut-associated bifidobacteria suggest the coevolution of the bacteria with humans.
Authors:
Sadaki Asakuma; Emi Hatakeyama; Tadasu Urashima; Erina Yoshida; Takane Katayama; Kenji Yamamoto; Hidehiko Kumagai; Hisashi Ashida; Junko Hirose; Motomitsu Kitaoka
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-08-09
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  286     ISSN:  1083-351X     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-03     Completed Date:  2011-12-09     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  34583-92     Citation Subset:  IM    
Affiliation:
National Agricultural Research Center for the Hokkaido Region, Sapporo, Hokkaido 062-8555, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/JF332149;  JF332150;  JF332151
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MeSH Terms
Descriptor/Qualifier:
Adult
Bifidobacteriales Infections / microbiology
Bifidobacterium / metabolism*
Carbohydrates / chemistry
Chromatography, High Pressure Liquid / methods
Female
Gastrointestinal Tract / microbiology
Humans
Infant, Newborn
Intestines / microbiology*
Japan
Milk, Human / metabolism*
Molecular Sequence Data
Monosaccharides
Oligosaccharides / chemistry,  metabolism*
Time Factors
Chemical
Reg. No./Substance:
0/Carbohydrates; 0/Monosaccharides; 0/Oligosaccharides
Comments/Corrections

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