| Physiology of Saccharomyces cerevisiae during cell cycle oscillations. | |
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MedLine Citation:
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PMID: 8987629 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Synchronized populations of Saccharomyces cerevisiae CBS 426 are characterized by autonomous oscillations of process variables. CO2 evolution rate, O2 uptake rate and heat production rate varied by a factor of 2 for a continuous culture grown at a dilution rate of 0.10 h-1. Elemental analysis showed that the carbon mass fraction of biomass did not change. Since the reactor is not at steady state, the elemental and energy balances were calculated on cumulated quantities, i.e. the integral of the reaction rates. It was possible to show that carbon, degree of reduction and energy balances matched. Application of simple mass balance principles for non-steady state systems indicated that oscillations were basically characterized by changes in biomass production rate. In addition, the amount of intermediates, e.g. ethanol or acetate, produced or consumed was negligible. Growth rate was low during the S-phase (0.075 h-1) and high during the G2, M and G1 phases (0.125 h-1) for a constant dilution rate of 0.10 h-1. However, nitrogen, ash, sulfur and potassium content showed systematic increases during the S-phase (bud initiation). Cell component analyses showed that changes in cellular fractions during oscillations (storage carbohydrate content decreased during the S-phase) were due to changes in production rates, particularly for protein and carbohydrates. Nevertheless, using the data evaluation techniques for dynamic systems presented here, it was shown that storage carbohydrates are not consumed during the S-phase. Only the synthesis rate of the different cell components changed depending on position in cell cycle. The growth process may be divided into two phenomena: the formation of new cells during mitosis with a low yield, and size increase of new born cells with high yield. Both kinetic and stoichiometric coefficients varied with the position in the oscillation: the results showed that biomass structure changed and that specific growth rate, as well as biomass yield, varied by +/- 25% during the oscillation. |
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Authors:
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P Duboc; I Marison; U von Stockar |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biotechnology Volume: 51 ISSN: 0168-1656 ISO Abbreviation: J. Biotechnol. Publication Date: 1996 Oct |
Date Detail:
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Created Date: 1997-02-13 Completed Date: 1997-02-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8411927 Medline TA: J Biotechnol Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 57-72 Citation Subset: B |
Affiliation:
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Institute of Chemical Engineering, Swiss Federal Institute of Technology, Lausanne, Switzerland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetic Acid
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metabolism Biomass Bioreactors Biotechnology Carbon Dioxide / metabolism Cell Cycle Energy Metabolism Ethanol / metabolism Macromolecular Substances Models, Biological Oxygen Consumption Saccharomyces cerevisiae / cytology*, growth & development, physiology* |
| Chemical | |
Reg. No./Substance:
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0/Macromolecular Substances; 124-38-9/Carbon Dioxide; 64-17-5/Ethanol; 64-19-7/Acetic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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