Document Detail

Physiological studies on the utilization of oleic acid by Saccharomyces cerevisiae in relation to microbody development.
MedLine Citation:
PMID:  1783286     Owner:  NLM     Status:  MEDLINE    
We studied the influence of specific growth conditions on the induction of beta-oxidation enzymes and rate of microbody proliferation in S. cerevisiae by oleic acid. Of all conditions tested, highest enzyme levels and microbody numbers were achieved in glucose-limited continuous cultures, supplemented with oleic acids second carbon source. Comparable enzyme levels were observed in identical cultures of peroxisome-deficient (pas) mutants of S. cerevisiae. These experiments showed chemostat cultures on glucose/oleic acid mixtures to be a method of choice for future studies on microbody biogenesis/assembly in constructed, conditional pas mutants.
M E Evers; J Höhfeld; W H Kunau; W Harder; M Veenhuis
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FEMS microbiology letters     Volume:  69     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  1991 Dec 
Date Detail:
Created Date:  1992-03-16     Completed Date:  1992-03-16     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  73-8     Citation Subset:  IM    
Laboratory for Electron Microscopy, University of Groningen, Haren, The Netherlands.
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MeSH Terms
Cell Division
Genes, Fungal
Glucose / metabolism
Microbodies / physiology*,  ultrastructure
Oleic Acid
Oleic Acids / physiology*
Promoter Regions, Genetic
Saccharomyces cerevisiae / genetics,  growth & development,  physiology*
Transformation, Genetic
Reg. No./Substance:
0/Oleic Acids; 112-80-1/Oleic Acid; 50-99-7/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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