Document Detail


Physiological studies of the interaction between opsin and chromophore in rod and cone visual pigments.
MedLine Citation:
PMID:  20552424     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The visual pigment in vertebrate photoreceptors is a G protein-coupled receptor that consists of a protein, opsin, covalently attached to a chromophore, 11-cis-retinal. Activation of the visual pigment by light triggers a transduction cascade that produces experimentally measurable electrical responses in photoreceptors. The interactions between opsin and chromophore can be investigated with electrophysiologial recordings in intact amphibian and mouse rod and cone photoreceptor cells. Here we describe methods for substituting the native chromophore with various chromophore analogs to investigate how specific parts of the chromophore affect the signaling properties of the visual pigment and the function of photoreceptors. We also describe methods for genetically substituting the native rod opsin gene with cone opsins or with mutant rod opsins to investigate and compare their signaling properties. These methods are useful not only for understanding the relation between the properties of visual pigments and the function of photoreceptors but also for understanding the mechanisms by which mutations in rod opsin produce night blindness and other visual disorders.
Authors:
Vladimir J Kefalov; M Carter Cornwall; Gordon L Fain
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  652     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2010  
Date Detail:
Created Date:  2010-06-16     Completed Date:  2010-09-09     Revised Date:  2014-09-15    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  95-114     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cone Opsins / metabolism*
Humans
Mice
Photoreceptor Cells, Vertebrate / metabolism*
Protein Binding
Retinaldehyde / analogs & derivatives,  metabolism*
Rod Opsins / metabolism*
Grant Support
ID/Acronym/Agency:
R01 EY001157/EY/NEI NIH HHS; R01 EY019312/EY/NEI NIH HHS; R21 EY019543/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/Cone Opsins; 0/Rod Opsins; RR725D715M/Retinaldehyde
Comments/Corrections

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