Document Detail

Physiological responses of wild type and putrescine-overproducing transgenic cells of poplar to variations in the form and concentration of nitrogen in the medium.
MedLine Citation:
PMID:  14996659     Owner:  NLM     Status:  MEDLINE    
We determined: (a) the physiological consequences of overproduction of putrescine in transgenic poplar (Populus nigra x maximoviczii) cells expressing an ornithine decarboxylase transgene; and (b) effects of variation in nitrogen (N) concentration of the medium on cellular polyamine concentration in transgenic and non-transgenic cells. Cells grown in the presence of supplemental (to the normal concentrations of N sources in the growth medium) and reduced amounts of NH4NO3 and KNO3 were used to study effects on membrane permeability, mitochondrial respiratory activity, protein accumulation, growth rates and changes in cellular polyamine concentration. The N concentration of the MS medium was not a limiting factor for continued overproduction of putrescine in transgenic cells. However, continued supplies of NH4+ and NO3- were required to maintain homeostatic amounts of putrescine in both cell lines. The presence of high amounts of putrescine in transgenic cells had significant effects on the physiological parameters measured. Compared with non-transgenic cells, transgenic cells had greater plasma membrane permeability, less tolerance to NH4NO3, more tolerance to KNO3, and accumulated higher amounts of soluble protein.
Rakesh Minocha; Jae Soon Lee; Stephanie Long; Pratiksha Bhatnagar; Subhash C Minocha
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Tree physiology     Volume:  24     ISSN:  0829-318X     ISO Abbreviation:  Tree Physiol.     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-03-03     Completed Date:  2005-12-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100955338     Medline TA:  Tree Physiol     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  551-60     Citation Subset:  IM    
USDA Forest Service, Northeastern Research Station, P.O. Box 640, Durham, NH 03824, USA.
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MeSH Terms
Cells, Cultured
Culture Media
Nitrogen / metabolism
Plants, Genetically Modified / cytology,  genetics,  physiology*
Populus / cytology,  genetics,  physiology*
Putrescine / biosynthesis*
Trees / cytology,  genetics,  physiology*
Reg. No./Substance:
0/Culture Media; 110-60-1/Putrescine; 7727-37-9/Nitrogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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