| Physiological requirements for carbonate precipitation during biofilm development of Bacillus subtilis etfA mutant. | |
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MedLine Citation:
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PMID: 20059546 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Although the implications of calcium carbonate (CaCO(3)) precipitation by microorganisms in natural environments are quite relevant, the physiology and genetics of this phenomenon are poorly understood. We have chosen Bacillus subtilis 168 as our model to study which physiological aspects are associated with CaCO(3) (calcite) formation during biofilm development when grown on precipitation medium. A B. subtilis eftA mutant named FBC5 impaired in calcite precipitation was used for comparative studies. Our results demonstrate that inactivation of etfA causes a decrease in the pH of the precipitation medium during biofilm development. Further analysis demonstrated that eftA extrudes an excess of 0.7 mol H(+) L(-1) with respect to B. subtilis 168 strain. Using media buffered at different pH values, we were able to control calcite formation. Because etfA encodes the alpha-subunit of a putative flavoprotein involved in fatty acid metabolism, we compared the intracellular levels of NADH among strains. Our physiological assay showed that FBC5 accumulated up to 32 times more NADH than the wild-type strain. We propose that the accumulation of NADH causes a deregulation in the generation of the proton motive force (DeltamicroH(+)) in FBC5 producing the acidification. |
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Authors:
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Massimiliano Marvasi; Pieter T Visscher; Brunella Perito; Giorgio Mastromei; Lilliam Casillas-Martínez |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2009-11-03 |
Journal Detail:
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Title: FEMS microbiology ecology Volume: 71 ISSN: 1574-6941 ISO Abbreviation: FEMS Microbiol. Ecol. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-05-12 Completed Date: 2010-08-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8901229 Medline TA: FEMS Microbiol Ecol Country: England |
Other Details:
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Languages: eng Pagination: 341-50 Citation Subset: IM |
Affiliation:
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Biology Department, University of Puerto Rico-Humacao, Humacao, PR 00791, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacillus subtilis
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growth & development,
physiology* Bacterial Proteins / genetics, metabolism* Biofilms / growth & development* Calcium Carbonate / metabolism* Crystallization Electron-Transferring Flavoproteins / genetics, metabolism* Hydrogen-Ion Concentration NAD / metabolism Protons Spectroscopy, Fourier Transform Infrared |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Electron-Transferring Flavoproteins; 0/Protons; 471-34-1/Calcium Carbonate; 53-84-9/NAD |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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