Document Detail

Physiological hyperinsulinemia inhibits myocardial protein degradation in vivo in the canine heart.
MedLine Citation:
PMID:  1628395     Owner:  NLM     Status:  MEDLINE    
Myocardial protein turnover in vivo was examined in anesthetized dogs following a 16- or 36-hour fast and again during a hyperinsulinemic (2 mU/kg per minute) euglycemic clamp with or without amino acid replacement or during saline infusion. We measured myocardial phenylalanine balance and rates of protein synthesis and degradation, using the extraction of intravenously infused L-[ring-2,6-3H]phenylalanine and the dilution of its specific activity across the heart at isotopic steady state. After both a 16-hour (n = 19) and 36-hour fast (n = 10), there was net myocardial release of phenylalanine indicated by the negative balances for phenylalanine of -52 +/- 9 (p less than 0.001) and -38 +/- 9 (p less than 0.005) nmol/min, respectively. Overall, the basal rate of myocardial protein degradation was lower in the 36-hour-fasted animals (81 +/- 13 versus 121 +/- 12 nmol/min, p less than 0.05). Myocardial phenylalanine balance and rates of protein synthesis and degradation did not change during insulin and glucose infusion in the 36-hour-fasted animals (n = 10). In these animals, there was a 30-40% decline in plasma amino acid concentrations, including branched chain (p less than 0.001) and essential amino acids (p less than 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)
L H Young; D M Dahl; D Rauner; E J Barrett
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Circulation research     Volume:  71     ISSN:  0009-7330     ISO Abbreviation:  Circ. Res.     Publication Date:  1992 Aug 
Date Detail:
Created Date:  1992-08-17     Completed Date:  1992-08-17     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0047103     Medline TA:  Circ Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  393-400     Citation Subset:  IM    
Department of Internal Medicine, Yale University School of Medicine, New Haven, Conn. 06510.
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MeSH Terms
Amino Acids / blood
Blood Glucose / analysis
Coronary Circulation
Glucose / metabolism
Infusions, Intravenous
Insulin / administration & dosage,  blood*
Muscle Proteins / metabolism*
Myocardium / metabolism*
Phenylalanine / metabolism
Time Factors
Grant Support
Reg. No./Substance:
0/Amino Acids; 0/Blood Glucose; 0/Muscle Proteins; 11061-68-0/Insulin; 50-99-7/Glucose; 63-91-2/Phenylalanine

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