Document Detail


Physiological and histological characterisation of a pig kidney in vitro perfusion model for xenotransplantation studies.
MedLine Citation:
PMID:  8837254     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A pig kidney perfusion model aimed for use in immunological and physiological xenotransplantation research has been developed. Organ viability was characterised by clearance studies, functional response to hormones/diureticum and by light microscopical examination. The pig kidney was perfused in a specially designed plexiglass chamber, using a roller pump and a small membrane oxygenator (O2/CO2, 95/5). The recirculating perfusate used was autologous pig blood diluted by Tyrodes solution to a hematocrit of 30%, at a total starting volume of 600-650 ml. The temperature was 37 degrees C. It was crucial for good organ function that the nephrectomy operating time, as well as the warm (1-2 min) and cold ischemia (average 43 min) times were minimized. The average total perfusion time was 151 minutes. Physiological parameters were measured during 10-15 minute periods at average times of 40, 63, 88 and 142 minutes. The clearance values of inulin in these periods were 54 +/- 13, 59 +/- 15, 48 +/- 23, 27 +/- 5 and for PAH; 103 +/- 14, 121 +/- 14, 106 +/- 30, 114 +/- 34 ml/min/100 g tissue weight. The plasma flows were 123 +/- 12, 155 +/- 17, 136 +/- 36 and 206 +/- 57 ml/min/100 g. The injection of 0.5 micrograms of alpha ANP to the perfusate resulted in a significant decrease in vascular resistance, and increase in urine production (+107%), as well as sodium (+112%) and potassium (+46%) excretion. Ten mg furosemide doubled the urine production and sodium excretion, while potassium excretion increased marginally. The number of leucocytes decreased by 39% during the perfusion, while the platelet count was unaffected. Light microscopy of the renal tissue after termination of the experiments revealed endothelial damage to variable extent. Loss of endothelial cells was most obvious at the level of arcuate and interlobular arteries, while the endothelium was intact in larger arteries and veins. Accumulation of polymorphonuclear granulocytes was found predominantly in the peritubular vessels, and to a lesser degree in the cortical venules. In the tubular cells, only minimal epithelial swelling and irregular cytoplasmic vacuolisation was found. Thus, a good functional viability can be maintained during 2 hours in vitro perfusion, although a decline in function as well as structural damage can be seen at the end of the experiment.
Authors:
M E Breimer; C T Svalander; B Haraldsson; S Björck
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Scandinavian journal of urology and nephrology     Volume:  30     ISSN:  0036-5599     ISO Abbreviation:  Scand. J. Urol. Nephrol.     Publication Date:  1996 Jun 
Date Detail:
Created Date:  1997-03-11     Completed Date:  1997-03-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0114501     Medline TA:  Scand J Urol Nephrol     Country:  SWEDEN    
Other Details:
Languages:  eng     Pagination:  213-21     Citation Subset:  IM    
Affiliation:
Department of Surgery (Transplantation division), University of Göteborg, Sahlgrens University Hospital, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Animals
Atrial Natriuretic Factor / pharmacology
Endothelium / pathology
Female
Furosemide / pharmacology
Inulin / metabolism
Kidney / pathology*,  physiology*
Kidney Transplantation*
Leukocyte Count
Male
Neutrophils / pathology
Perfusion
Platelet Count
Potassium / urine
Renal Plasma Flow
Sodium / urine
Swine
Temperature
Time Factors
Tissue Survival
Transplantation, Heterologous
Urine
Vascular Resistance / drug effects
Chemical
Reg. No./Substance:
54-31-9/Furosemide; 7440-09-7/Potassium; 7440-23-5/Sodium; 85637-73-6/Atrial Natriuretic Factor; 9005-80-5/Inulin

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