Document Detail


Physiological fluctuations in brain temperature as a factor affecting electrochemical evaluations of extracellular glutamate and glucose in behavioral experiments.
MedLine Citation:
PMID:  23448428     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The rate of any chemical reaction or process occurring in the brain depends on temperature. While it is commonly believed that brain temperature is a stable, tightly regulated homeostatic parameter, it fluctuates within 1-4 °C following exposure to salient arousing stimuli and neuroactive drugs, and during different behaviors. These temperature fluctuations should affect neural activity and neural functions, but the extent of this influence on neurochemical measurements in brain tissue of freely moving animals remains unclear. In this Review, we present the results of amperometric evaluations of extracellular glutamate and glucose in awake, behaving rats and discuss how naturally occurring fluctuations in brain temperature affect these measurements. While this temperature contribution appears to be insignificant for glucose because its extracellular concentrations are large, it is a serious factor for electrochemical evaluations of glutamate, which is present in brain tissue at much lower levels, showing smaller phasic fluctuations. We further discuss experimental strategies for controlling the nonspecific chemical and physical contributions to electrochemical currents detected by enzyme-based biosensors to provide greater selectivity and reliability of neurochemical measurements in behaving animals.
Authors:
Eugene A Kiyatkin; Ken T Wakabayashi; Magalie Lenoir
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural; Review     Date:  2013-03-14
Journal Detail:
Title:  ACS chemical neuroscience     Volume:  4     ISSN:  1948-7193     ISO Abbreviation:  ACS Chem Neurosci     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-07-18     Completed Date:  2013-10-22     Revised Date:  2014-05-16    
Medline Journal Info:
Nlm Unique ID:  101525337     Medline TA:  ACS Chem Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  652-65     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Body Temperature / physiology*
Brain / physiology*
Electrochemical Techniques
Extracellular Space / chemistry*
Glucose / analysis*
Glutamic Acid / analysis*
Rats
Grant Support
ID/Acronym/Agency:
T32 DA007281/DA/NIDA NIH HHS
Chemical
Reg. No./Substance:
3KX376GY7L/Glutamic Acid; IY9XDZ35W2/Glucose
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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