| Physiological evidence for the discrimination of L-arginine from structural analogues by the zebrafish olfactory system. | |
| | |
MedLine Citation:
|
PMID: 10601449 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Although it is generally assumed that fish are capable of discriminating amino acid odorants on the basis of differences in side-chain structure, less is known about their ability to discriminate amino acids with modifications to alpha-carboxyl and alpha-amino groups. In this study, the ability of the zebrafish olfactory system to detect and presumably discriminate analogues of the basic amino acid Arg was assessed, by using cross-adaptation and activity-dependent labeling techniques. Electrophysiological recordings established that esterification (L-arginine methyl ester; AME) or deletion (agmatine or amino-4-guanidobutane; AGB) of the alpha-carboxyl group yielded odorants more potent than Arg, whereas deletion of the alpha-amino group (L-argininic acid; AA) yielded a less potent analogue. In cross-adaptation experiments, no test-competitor odorant combination yielded complete cross-adaptation, suggesting the detection of these Arg analogues by multiple odorant receptors (ORs) with partially nonoverlapping specificities. Activity-dependent immunocytochemical labeling of olfactory receptor neurons supported this conclusion. AGB, an ion-channel-permeant probe (and odorant), labeled 4.9 +/- 0.4% (n = 24) of sensory epithelium, whereas the addition of Arg, 1-ethylguanidine sulfate, L-alpha-amino-beta-guanidinopropionate, or AME to AGB resulted in a significant elevation of labeling (8-14%). This study provides evidence that the olfactory system has the potential to discriminate among amino acid odorants with modified alpha-carboxyl and alpha-amino groups. |
| | |
Authors:
|
D L Lipschitz; W C Michel |
Related Documents
:
|
22856559 - Inactivation of salmonella on tomato stem scars by edible chitosan and organic acid coa... 1756619 - Species-specific antibodies of tetrahymena acid alpha-glucosidase. 18497929 - Organometal additions to alpha-iminoesters: n-alkylation via umpolung. 11316579 - Protection by thiols of the mitochondrial complexes from 4-hydroxy-2-nonenal. 10080389 - Alternative 2-keto acid oxidoreductase activities in trichomonas vaginalis. 11132839 - Proteolysis of alphas-casein as a marker of grana padano cheese ripening. 10368179 - Structure of a plant cell wall fragment complexed to pectate lyase c. 20797869 - Synthesis of cyclic di-nucleotidic acids as potential inhibitors targeting diguanylate ... 11935059 - Prognostic significance of uric acid serum concentration in patients with acute ischemi... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Journal of neurophysiology Volume: 82 ISSN: 0022-3077 ISO Abbreviation: J. Neurophysiol. Publication Date: 1999 Dec |
Date Detail:
|
Created Date: 2000-02-03 Completed Date: 2000-02-03 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0375404 Medline TA: J Neurophysiol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 3160-7 Citation Subset: IM |
Affiliation:
|
Department of Physiology, University of Utah School of Medicine, Salt Lake City, Utah 84108-1270, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adaptation, Physiological
/
physiology Animals Arginine / analogs & derivatives*, pharmacology* Discrimination (Psychology) / physiology* Electrophysiology Female Image Processing, Computer-Assisted Immunohistochemistry Male Odors Olfactory Receptor Neurons / physiology* Smell / physiology* Structure-Activity Relationship Zebrafish |
| Grant Support | |
ID/Acronym/Agency:
|
5PO1-NS-07938/NS/NINDS NIH HHS; DC-01418/DC/NIDCD NIH HHS |
| Chemical | |
Reg. No./Substance:
|
74-79-3/Arginine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Processing of gustatory information by spiking local interneurons in the locust.
Next Document: Gentamicin blocks both fast and slow effects of olivocochlear activation in anesthetized guinea pigs...