Document Detail


Physiological evidence for the discrimination of L-arginine from structural analogues by the zebrafish olfactory system.
MedLine Citation:
PMID:  10601449     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although it is generally assumed that fish are capable of discriminating amino acid odorants on the basis of differences in side-chain structure, less is known about their ability to discriminate amino acids with modifications to alpha-carboxyl and alpha-amino groups. In this study, the ability of the zebrafish olfactory system to detect and presumably discriminate analogues of the basic amino acid Arg was assessed, by using cross-adaptation and activity-dependent labeling techniques. Electrophysiological recordings established that esterification (L-arginine methyl ester; AME) or deletion (agmatine or amino-4-guanidobutane; AGB) of the alpha-carboxyl group yielded odorants more potent than Arg, whereas deletion of the alpha-amino group (L-argininic acid; AA) yielded a less potent analogue. In cross-adaptation experiments, no test-competitor odorant combination yielded complete cross-adaptation, suggesting the detection of these Arg analogues by multiple odorant receptors (ORs) with partially nonoverlapping specificities. Activity-dependent immunocytochemical labeling of olfactory receptor neurons supported this conclusion. AGB, an ion-channel-permeant probe (and odorant), labeled 4.9 +/- 0.4% (n = 24) of sensory epithelium, whereas the addition of Arg, 1-ethylguanidine sulfate, L-alpha-amino-beta-guanidinopropionate, or AME to AGB resulted in a significant elevation of labeling (8-14%). This study provides evidence that the olfactory system has the potential to discriminate among amino acid odorants with modified alpha-carboxyl and alpha-amino groups.
Authors:
D L Lipschitz; W C Michel
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of neurophysiology     Volume:  82     ISSN:  0022-3077     ISO Abbreviation:  J. Neurophysiol.     Publication Date:  1999 Dec 
Date Detail:
Created Date:  2000-02-03     Completed Date:  2000-02-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375404     Medline TA:  J Neurophysiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3160-7     Citation Subset:  IM    
Affiliation:
Department of Physiology, University of Utah School of Medicine, Salt Lake City, Utah 84108-1270, USA.
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MeSH Terms
Descriptor/Qualifier:
Adaptation, Physiological / physiology
Animals
Arginine / analogs & derivatives*,  pharmacology*
Discrimination (Psychology) / physiology*
Electrophysiology
Female
Image Processing, Computer-Assisted
Immunohistochemistry
Male
Odors
Olfactory Receptor Neurons / physiology*
Smell / physiology*
Structure-Activity Relationship
Zebrafish
Grant Support
ID/Acronym/Agency:
5PO1-NS-07938/NS/NINDS NIH HHS; DC-01418/DC/NIDCD NIH HHS
Chemical
Reg. No./Substance:
74-79-3/Arginine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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