Document Detail


Physiological and biochemical performances of menthol-induced aposymbiotic corals.
MedLine Citation:
PMID:  23029512     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The unique mutualism between corals and their photosynthetic zooxanthellae (Symbiodinium spp.) is the driving force behind functional assemblages of coral reefs. However, the respective roles of hosts and Symbiodinium in this endosymbiotic association, particularly in response to environmental challenges (e.g., high sea surface temperatures), remain unsettled. One of the key obstacles is to produce and maintain aposymbiotic coral hosts for experimental purposes. In this study, a simple and gentle protocol to generate aposymbiotic coral hosts (Isopora palifera and Stylophora pistillata) was developed using repeated incubation in menthol/artificial seawater (ASW) medium under light and in ASW in darkness, which depleted more than 99% of Symbiodinium from the host within 4∼8 days. As indicated by the respiration rate, energy metabolism (by malate dehydrogenase activity), and nitrogen metabolism (by glutamate dehydrogenase activity and profiles of free amino acids), the physiological and biochemical performances of the menthol-induced aposymbiotic corals were comparable to their symbiotic counterparts without nutrient supplementation (e.g., for Stylophora) or with a nutrient supplement containing glycerol, vitamins, and a host mimic of free amino acid mixture (e.g., for Isopora). Differences in biochemical responses to menthol-induced bleaching between Stylophora and Isopora were attributed to the former digesting Symbiodinium rather than expelling the algae live as found in the latter species. Our studies showed that menthol could successfully bleach corals and provided aposymbiotic corals for further exploration of coral-alga symbioses.
Authors:
Jih-Terng Wang; Yi-Yun Chen; Kwee Siong Tew; Pei-Jei Meng; Chaolun A Chen
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-09-27
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-10-02     Completed Date:  2013-02-21     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e46406     Citation Subset:  IM    
Affiliation:
Graduate Institute of Biotechnology, Tajen University, Pingtung, Taiwan. jtw@mail.tajen.edu.tw
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MeSH Terms
Descriptor/Qualifier:
Animals
Anthozoa / drug effects,  physiology*
Cell Respiration / physiology
Coral Reefs
Dinoflagellida / drug effects,  physiology*
Glutamate Dehydrogenase / metabolism
Light
Malate Dehydrogenase / metabolism
Menthol / pharmacology*
Oceans and Seas
Photosynthesis
Seawater
Species Specificity
Symbiosis
Temperature
Chemical
Reg. No./Substance:
1490-04-6/Menthol; EC 1.1.1.37/Malate Dehydrogenase; EC 1.4.1.2/Glutamate Dehydrogenase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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