| Physiological and biochemical performances of menthol-induced aposymbiotic corals. | |
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MedLine Citation:
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PMID: 23029512 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The unique mutualism between corals and their photosynthetic zooxanthellae (Symbiodinium spp.) is the driving force behind functional assemblages of coral reefs. However, the respective roles of hosts and Symbiodinium in this endosymbiotic association, particularly in response to environmental challenges (e.g., high sea surface temperatures), remain unsettled. One of the key obstacles is to produce and maintain aposymbiotic coral hosts for experimental purposes. In this study, a simple and gentle protocol to generate aposymbiotic coral hosts (Isopora palifera and Stylophora pistillata) was developed using repeated incubation in menthol/artificial seawater (ASW) medium under light and in ASW in darkness, which depleted more than 99% of Symbiodinium from the host within 4∼8 days. As indicated by the respiration rate, energy metabolism (by malate dehydrogenase activity), and nitrogen metabolism (by glutamate dehydrogenase activity and profiles of free amino acids), the physiological and biochemical performances of the menthol-induced aposymbiotic corals were comparable to their symbiotic counterparts without nutrient supplementation (e.g., for Stylophora) or with a nutrient supplement containing glycerol, vitamins, and a host mimic of free amino acid mixture (e.g., for Isopora). Differences in biochemical responses to menthol-induced bleaching between Stylophora and Isopora were attributed to the former digesting Symbiodinium rather than expelling the algae live as found in the latter species. Our studies showed that menthol could successfully bleach corals and provided aposymbiotic corals for further exploration of coral-alga symbioses. |
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Authors:
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Jih-Terng Wang; Yi-Yun Chen; Kwee Siong Tew; Pei-Jei Meng; Chaolun A Chen |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-09-27 |
Journal Detail:
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Title: PloS one Volume: 7 ISSN: 1932-6203 ISO Abbreviation: PLoS ONE Publication Date: 2012 |
Date Detail:
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Created Date: 2012-10-02 Completed Date: 2013-02-21 Revised Date: 2013-03-01 |
Medline Journal Info:
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Nlm Unique ID: 101285081 Medline TA: PLoS One Country: United States |
Other Details:
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Languages: eng Pagination: e46406 Citation Subset: IM |
Affiliation:
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Graduate Institute of Biotechnology, Tajen University, Pingtung, Taiwan. jtw@mail.tajen.edu.tw |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anthozoa / drug effects, physiology* Cell Respiration / physiology Coral Reefs Dinoflagellida / drug effects, physiology* Glutamate Dehydrogenase / metabolism Light Malate Dehydrogenase / metabolism Menthol / pharmacology* Oceans and Seas Photosynthesis Seawater Species Specificity Symbiosis Temperature |
| Chemical | |
Reg. No./Substance:
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1490-04-6/Menthol; EC 1.1.1.37/Malate Dehydrogenase; EC 1.4.1.2/Glutamate Dehydrogenase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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