Document Detail

Physiological behaviour of Saccharomyces cerevisiae in aerated fed-batch fermentation for high level production of bioethanol.
MedLine Citation:
PMID:  17005001     Owner:  NLM     Status:  MEDLINE    
Saccharomyces cerevisiae was able to produce 20% (v/v) of ethanol in 45 h in a fully aerated fed-batch process recently developed in our laboratory. A notable feature of this process was a production phase uncoupled to growth, the extent of which was critical for high-level ethanol production. As the level of production was found to be highly variable, we investigated on this high variability by means of a detailed physiological analysis of yeast cells in two fed-batch fermentations showing the most extreme behaviour. We found a massive leakage of intracellular metabolites into the growth medium which correlated with the drop of cell viability. The loss of viability was also found to be proportional to the reduction of plasma membrane phospholipids. Finally, the fed-batch processes with the longest uncoupling phase were characterized by induction of storage carbohydrates at the onset of this phase, whereas this metabolic event was not seen in processes with a short uncoupling phase. Taken together, our results suggested that reproducible high-level bioethanol production in aerated fed-batch processes may be linked to the ability of yeast cells to impede ethanol toxicity by triggering a metabolic remodelling reminiscent to that of cells entering a quiescent GO/G1 state.
Marlène Cot; Marie-Odile Loret; Jean François; Laurent Benbadis
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-09-28
Journal Detail:
Title:  FEMS yeast research     Volume:  7     ISSN:  1567-1356     ISO Abbreviation:  FEMS Yeast Res.     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2007-02-21     Completed Date:  2007-03-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101085384     Medline TA:  FEMS Yeast Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  22-32     Citation Subset:  IM    
Laboratoire de Biotechnologie & Bioprocédés, UMR-CNRS 5504, UMR-INRA792, Institut National des Sciences Appliquées, Avenue de Rangueil, Toulouse Cedex 04, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Adaptation, Physiological*
Culture Media
Ethanol / metabolism*
Gene Expression Regulation, Fungal
Industrial Microbiology / methods
Saccharomyces cerevisiae / growth & development,  metabolism,  physiology*
Reg. No./Substance:
0/Culture Media; 64-17-5/Ethanol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Coping with the isotretinoin registry.
Next Document:  Splice variants of human FOXP3 are functional inhibitors of human CD4+ T-cell activation.