Document Detail

Physiological T cell activation starts and propagates in lipid rafts.
MedLine Citation:
PMID:  14757363     Owner:  NLM     Status:  MEDLINE    
Lipid rafts are plasma membrane compartments enriched in key signaling molecules. We have previously shown that in T lymphocytes anti-CD3 stimulation is insensitive to cholesterol extraction by methyl-beta-cyclodextrin (MbetaCD), suggesting that anti-CD3 induced signal transduction is independent of raft integrity. Here we show that, in contrast to T cell stimulation by anti-CD3 antibodies, T cell activation by a physiological ligand is mediated by signaling events taking place in lipid raft. Indeed, cholesterol depletion by MbetaCD resulted in reduced T cell activation in response to Epstein Barr Virus (EBV)-transformed B cells pulsed with a bacterial superantigen. Moreover, T cell stimulation by pulsed EBV-B cells, but not by anti-CD3 antibodies, induced recruitment of active Lck in detergent-resistant membranes, where the signal transduction is organized and amplified.
Paola Pizzo; Emanuele Giurisato; Anna Bigsten; Maristella Tassi; Regina Tavano; Andrey Shaw; Antonella Viola
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunology letters     Volume:  91     ISSN:  0165-2478     ISO Abbreviation:  Immunol. Lett.     Publication Date:  2004 Jan 
Date Detail:
Created Date:  2004-02-03     Completed Date:  2004-03-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7910006     Medline TA:  Immunol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  3-9     Citation Subset:  IM    
Department of Biomedical Sciences, University of Padova, via G Colombo 3, 35121 Padova, Italy.
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MeSH Terms
Antibodies / immunology
Antigens, CD3 / immunology
Calcium / metabolism
Cholesterol / metabolism
Jurkat Cells
Lymphocyte Activation / immunology,  physiology*
Membrane Microdomains / immunology,  physiology*
Receptors, Antigen, T-Cell / immunology,  physiology
T-Lymphocytes / immunology,  physiology*
Reg. No./Substance:
0/Antibodies; 0/Antigens, CD3; 0/Receptors, Antigen, T-Cell; 57-88-5/Cholesterol; 7440-70-2/Calcium

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