| Physiologic pulsatile flow bioreactor conditioning of poly(ethylene glycol)-based tissue engineered vascular grafts. | |
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MedLine Citation:
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PMID: 17180465 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mechanical conditioning represents a potential means to enhance the biochemical and biomechanical properties of tissue engineered vascular grafts (TEVGs). A pulsatile flow bioreactor was developed to allow shear and pulsatile stimulation of TEVGs. Physiological 120 mmHg/80 mmHg peak-to-trough pressure waveforms can be produced at both fetal and adult heart rates. Flow rates of 2 mL/sec, representative of flow through small diameter blood vessels, can be generated, resulting in a mean wall shear stress of approximately 6 dynes/cm(2) within the 3 mm ID constructs. When combined with non-thrombogenic poly(ethylene glycol) (PEG)-based hydrogels, which have tunable mechanical properties and tailorable biofunctionality, the bioreactor represents a flexible platform for exploring the impact of controlled biochemical and biomechanical stimuli on vascular graft cells. In the present study, the utility of this combined approach for improving TEVG outcome was investigated by encapsulating 10T-1/2 mouse smooth muscle progenitor cells within PEG-based hydrogels containing an adhesive ligand (RGDS) and a collagenase degradable sequence (LGPA). Constructs subjected to 7 weeks of biomechanical conditioning had significantly higher collagen levels and improved moduli relative to those grown under static conditions. |
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Authors:
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Mariah S Hahn; Melissa K McHale; Eva Wang; Rachael H Schmedlen; Jennifer L West |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2006-12-16 |
Journal Detail:
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Title: Annals of biomedical engineering Volume: 35 ISSN: 0090-6964 ISO Abbreviation: Ann Biomed Eng Publication Date: 2007 Feb |
Date Detail:
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Created Date: 2007-01-15 Completed Date: 2007-03-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0361512 Medline TA: Ann Biomed Eng Country: United States |
Other Details:
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Languages: eng Pagination: 190-200 Citation Subset: IM |
Affiliation:
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Rice University, Department of Bioengineering, Houston, TX, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bioprosthesis Bioreactors* Blood Flow Velocity / physiology Blood Vessel Prosthesis* Blood Vessels / cytology*, growth & development* Cell Culture Techniques / instrumentation, methods Cell Line Cell Proliferation Equipment Design Equipment Failure Analysis Mechanotransduction, Cellular / physiology Mice Microfluidic Analytical Techniques / instrumentation, methods Myocytes, Smooth Muscle / cytology*, physiology* Polyethylene Glycols / chemistry Pulsatile Flow / physiology Tissue Engineering / instrumentation*, methods |
| Chemical | |
Reg. No./Substance:
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0/Polyethylene Glycols |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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