| Physiologic levels of epidermal growth factor in saliva stimulate cell migration of an oral epithelial cell line, HO-1-N-1. | |
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MedLine Citation:
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PMID: 12013556 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An oral epithelial cell line derived from buccal mucosa squamous cell carcinoma, HO-1-N-1, was used to elucidate the role of epidermal growth factor (EGF) in saliva on wound healing of the oral mucosa. The effects of EGF on DNA synthesis, and cell migration was studied and the related signal transduction pathways examined. DNA synthesis by HO-1-N-1 cells was stimulated dose-dependently by 1-10 ng ml(-1) EGF, but significantly inhibited by addition of a PI3-K inhibitor (wortmannin), a p38 MAPK inhibitor (SB203580) or an MEKs inhibitor (PD98059). Cell migration was also accelerated by addition of 1-10 ng ml(-1) EGF; however, the migration rate was decreased to 30% by adding PD98059, to 40% by adding a tyrosine kinase inhibitor (herbimycin A), and to 60% by adding wortmannin or dexamethasone. These results indicate that the physiologic concentration of EGF in saliva may stimulate proliferation and migration of oral epithelial cells for wound healing, when the oral mucosa has been injured. Furthermore, this study revealed that EGF-stimulated signal transduction pathways for epithelial cell proliferation and cell migration are different. |
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Authors:
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Mitsuhiro Ohshima; Masashi Sato; Masako Ishikawa; Masao Maeno; Kichibee Otsuka |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of oral sciences Volume: 110 ISSN: 0909-8836 ISO Abbreviation: Eur. J. Oral Sci. Publication Date: 2002 Apr |
Date Detail:
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Created Date: 2002-05-15 Completed Date: 2002-08-12 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9504563 Medline TA: Eur J Oral Sci Country: Denmark |
Other Details:
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Languages: eng Pagination: 130-6 Citation Subset: D; IM |
Affiliation:
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Department of Biochemistry, Nihon University School of Dentistry, Tokyo, Japan. oshima-m@dent.nihon-u.ac.jp |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Androstadienes
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pharmacology Carcinoma, Squamous Cell Cell Division Cell Movement / drug effects, physiology* DNA / biosynthesis Dexamethasone / pharmacology Enzyme Inhibitors / pharmacology Epidermal Growth Factor / pharmacology, physiology* Epithelial Cells / physiology Glucocorticoids / pharmacology Humans Immunity, Mucosal / physiology* MAP Kinase Signaling System / drug effects Mouth Mucosa / physiology* Mouth Neoplasms Saliva / chemistry, physiology* Salivary Proteins and Peptides / physiology* Tumor Cells, Cultured Wound Healing / physiology* |
| Chemical | |
Reg. No./Substance:
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0/Androstadienes; 0/Enzyme Inhibitors; 0/Glucocorticoids; 0/Salivary Proteins and Peptides; 19545-26-7/wortmannin; 50-02-2/Dexamethasone; 62229-50-9/Epidermal Growth Factor; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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