| Physiologic determinants of endothelin concentrations in human saliva. | |
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MedLine Citation:
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PMID: 14633872 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Salivary endothelin (ET) concentrations have been shown to correlate with disease severity in patients with chronic heart failure (CHF). We undertook the present study to evaluate the stability of salivary ET under different handling conditions to assess its suitability as a biochemical marker in screening, diagnosis, and management of CHF. METHODS: Saliva samples were collected from healthy individuals and/or CHF patients, subjected to different handling conditions, and then stored at -80 degrees C until assayed by an ELISA for ET. RESULTS: Salivary ET concentrations showed a time-dependent increase during storage at room temperature. After 72 h of incubation at room temperature, ET increased approximately 2.8-fold (P = 0.03). Simultaneously, salivary big ET showed a time-dependent 11.2-fold decrease (P <0.0001). This activity was blocked by an ET-converting enzyme (ECE) inhibitor, suggesting that these changes were attributable to ECE-dependent cleavage of endogenous big ET in saliva. Ex vivo conversion was also observed when samples were stored at 4 degrees C, but the magnitude of these changes was markedly smaller (P <0.0001). Posture also affected salivary ET concentrations in CHF patients. With a change from supine to seated rest, salivary ET concentrations increased 1.5- and 1.8-fold after 20 and 40 min, respectively (P = 0.01). With a return to supine rest, salivary ET concentrations returned to baseline concentrations (P = 0.008). CONCLUSIONS: These data suggest that saliva sampling and handling conditions could markedly affect measurement of salivary ET. In particular, care should be taken to minimize ECE-dependent enzymatic conversion of endogenous big ET in saliva. |
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Authors:
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Sue Xiang; Rachel Denver; Michael Bailey; Henry Krum |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical chemistry Volume: 49 ISSN: 0009-9147 ISO Abbreviation: Clin. Chem. Publication Date: 2003 Dec |
Date Detail:
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Created Date: 2003-11-24 Completed Date: 2003-12-19 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9421549 Medline TA: Clin Chem Country: United States |
Other Details:
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Languages: eng Pagination: 2012-9 Citation Subset: IM |
Affiliation:
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Department of Medicine and Department of Epidemiology and Preventive Medicine, Monash University, Alfred Hospital, Melbourne, Victoria 3004, Australia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aspartic Acid Endopeptidases / antagonists & inhibitors, metabolism Biological Markers / analysis Chemiluminescent Measurements Chronic Disease Circadian Rhythm Endothelin-1 / analysis Endothelins / analysis*, metabolism Enzyme-Linked Immunosorbent Assay Heart Failure / diagnosis* Humans Metalloendopeptidases Middle Aged Posture Saliva / chemistry*, metabolism Specimen Handling Temperature |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Endothelin-1; 0/Endothelins; EC 3.4.23.-/Aspartic Acid Endopeptidases; EC 3.4.24.-/Metalloendopeptidases; EC 3.4.24.71/endothelin-converting enzyme |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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