Document Detail

Physiologic aging of mature porcine erythrocytes: effects of various metabolites, antimetabolites, and physical stressors.
MedLine Citation:
PMID:  3777639     Owner:  NLM     Status:  MEDLINE    
Red blood cells were collected from Landrace X Duroc pigs in pooled and single batches. The RBC were stored for 24 hours to 20 days and exposed to 1 or more chemical and physical stressors. The chemicals were pyruvate, lactate, inosine, concanavalin A-luminol-bovine serum albumin conjugate, hydrogen peroxide, L-mimosine, and 3-amino-L-tyrosine. Physical stressors included thermogenic microwave radiation (2,450 MHz, mean specific absorption rate of 91 W/kg) and conventional heating with hot air or hot-water bath. Heating erythrocytes to 43 C for 10 minutes with microwaves or hot air did not significantly (P greater than 0.05) increase hemolysis, compared with hemolysis of RBC at 4 C (controls). Pyruvate or lactate did not affect the RBC under these conditions. Hemolysis of cells coated with concanavalin A-luminol-bovine serum albumin and heated to 43 C for 10 minutes by use of microwaves or hot air was significantly (P less than 0.05 by Student's t test) greater (48%) than that of RBC at 4 C (controls). The method of heating or the presence of pyruvate, lactate, or inosine did not have a significant (P greater than 0.05) effect on hemolysis. Lysis of RBC (14 days after collection) coated with concanavalin A-luminol-bovine serum albumin and stored at 4 C was not significantly different (P greater than 0.05) than that of noncoated cells (6 days after collection) stored at 4 C (control). When RBC were heated 20 days after collection to 48 C for 30 minutes, using a hot water bath, hemolysis was 60.6% greater than that of control cells (4 C).(ABSTRACT TRUNCATED AT 250 WORDS)
J L Kiel; D N Erwin
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  American journal of veterinary research     Volume:  47     ISSN:  0002-9645     ISO Abbreviation:  Am. J. Vet. Res.     Publication Date:  1986 Oct 
Date Detail:
Created Date:  1986-12-09     Completed Date:  1986-12-09     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0375011     Medline TA:  Am J Vet Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2155-60     Citation Subset:  IM    
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MeSH Terms
Concanavalin A / pharmacology
Erythrocyte Aging* / drug effects
Erythrocytes / metabolism
Hot Temperature
Hydrogen Peroxide / pharmacology
Inosine / pharmacology
Lactates / pharmacology
Luminescent Measurements
Luminol / pharmacology
Mimosine / pharmacology
Pyruvates / pharmacology
Serum Albumin, Bovine / pharmacology
Swine / blood*
Time Factors
Tyrosine / analogs & derivatives,  pharmacology
Reg. No./Substance:
0/Lactates; 0/Pyruvates; 0/Serum Albumin, Bovine; 11028-71-0/Concanavalin A; 300-34-5/3-aminotyrosine; 500-44-7/Mimosine; 521-31-3/Luminol; 55520-40-6/Tyrosine; 58-63-9/Inosine; 7722-84-1/Hydrogen Peroxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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